Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au-etching of gold nanobipyramids and CIELab color space.

Talanta

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection & Key Lab of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China. Electronic address:

Published: January 2025

AI Article Synopsis

  • The study introduces a new paper-based system using acetylcholinesterase (AChE) and gold nanobipyramids (AuNBPs) for the on-site detection of organophosphorus pesticides (OPs) in food.
  • This system enables colorimetric analysis to indicate the presence of OPs, with impressive sensitivity and accuracy, allowing for detection at very low concentrations in vegetable and rice samples.
  • The portable design and reliability of the system show promise for practical applications in monitoring food safety and can be adapted for various other detection purposes.

Article Abstract

On-site quantitative detection of organophosphorus pesticides (OPs) is crucial for safeguarding food and public safety. This study presents a novel acetylcholinesterase (AChE)-mediated paper-based Au-etching of gold nanobipyramids (AuNBPs) system. The system employs a long-term storable AuNBPs-deposited nylon membrane embedded within a portable and temperature-controlled paper-based analytical device. This system, coupled with a colorimeter-based quantitative method, enables the development of a practical paper-based multicolor sensor (PMS) for on-site quantitative detection of three common OPs (paraoxon, dichlorvos, and trichlorfon). In the absence of OPs, AChE hydrolyzes acetylthiocholine to thiocholine, which reduces Au to Au. The presence of OPs inhibits AChE activity, thereby preserving Au to etch AuNBPs on nylon membranes, accompanied by multicolor changes. These color changes can be simply quantified by measuring the a∗ parameter of the CIELab color space using a portable colorimeter. Under optimal conditions, the PMS displayed eight OPs-corresponding color changes with a minimum detectable concentration of 1.0-10 μg/L (visual observation) and limits of detection of 0.8-7.2 μg/L (colorimeter) and 0.2-3.4 μg/L (UV-vis spectrometry). The PMS successfully determined the OPs in vegetable and rice samples with recoveries of 89.0-109 % and RSDs (n = 5) of <6 %. These results were consistent with those obtained using the HPLC-MS method. The PMS demonstrates excellent portability, AuNBPs stability, detection sensitivity, and reproducibility, making it a promising tool for the on-site quantitative detection of OPs residues in food. Furthermore, the paper-based etching system coupled with the colorimeter-based quantitative method provides a valuable reference to develop practical PMSs for various targets in diverse fields.

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Source
http://dx.doi.org/10.1016/j.talanta.2024.126925DOI Listing

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