Dysbiosis-activated IL-17-producing T cells promote skin immunopathological progression in mice deficient of the Notch ligand Jag1 in keratinocytes.

J Dermatol Sci

Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health Science Center San Antonio, TX, USA; Department of Medicine-Division of Dermatology and Cutaneous Surgery, University of Texas Health Science Center San Antonio, TX , USA. Electronic address:

Published: October 2024

Background: The Notch signaling pathway is an evolutionarily conserved regulatory cascade critical in skin development and homeostasis. Mice deficient of Notch signaling molecules have impaired skin and hair follicle development associated with local tissue inflammation. However, mechanisms underlying skin inflammation and pathology resulting from defective Notch signals are not well understood.

Objective: To dissect molecular and cellular mechanisms underlying development of skin immunopathology in mice defective of the Notch ligand Jagged-1 (Jag1).

Methods: We assessed involvement of microbiota and immune cell subsets in skin pathogenic symptoms in Foxn1Jag1 mice that were deficient of Jag1 in keratinocytes. We also used RNA-seq and 16S rRNA gene-seq analyses to identify molecular factors and bacterial species contributing to skin pathologic symptoms in Foxn1Jag1 mice.

Results: Compared to Jag1-sufficient littermate control mice, Foxn1Jag1 mice had specific expansion of IL-17a-producing T cells accompanying follicular and epidermal hyperkeratosis and cyst formation while antibody blockage of IL-17a reduced the skin pathology. RNA-sequencing and 16S rRNA gene-sequencing analyses revealed dysregulated immune responses and altered microbiota compositions in the skin of Foxn1Jag1 mice. Antibiotic treatment completely prevented over-activation of IL-17a-producing T cells and alleviated skin pathology in Foxn1Jag1 mice.

Conclusion: Dysbiosis-induced over-activation of IL-17a-producing T cells is critically involved in development of skin pathology in Foxn1Jag1 mice, establishing Foxn1Jag1 mice as a useful model to study pathogenesis and therapeutic targets in microbiota-IL-17-mediated skin inflammatory diseases such as hidradenitis suppurativa (HS) and psoriasis.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jdermsci.2024.09.001DOI Listing

Publication Analysis

Top Keywords

foxn1jag1 mice
20
skin
12
mice deficient
12
il-17a-producing cells
12
skin pathology
12
mice
9
deficient notch
8
notch ligand
8
jag1 keratinocytes
8
notch signaling
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!