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Multi-level fingerprinting and immune activity evaluation for polysaccharides from Dioscorea opposita Thunb. | LitMetric

To establish the quality control method of Dioscorea opposita Thunb., the multi-level fingerprinting of polysaccharides was established and the relationship between fingerprint and immune activity was analyzed. The two molecular weight segments Mw1 (1.38 × 10-1.63 × 10 Da) and Mw2 (3.27 × 10-4.37 × 10 Da), thirteen infrared absorption peaks (3399.26 cm, 2929.32 cm, 1631.78 cm, 1400.39 cm, 1351.80 cm, 1123.58 cm, 1024.76 cm, 931.53 cm, 854.76 cm, 760.43 cm, 708.14 cm, 616.47 cm, and 526.78 cm), and four monosaccharides (Man, Rha, GalA, and Glc) were used to evaluate the quality of Dioscorea opposita Thunb. The molecular weight fragments of Mw1, FT-IR absorption peaks of 1631.78 cm, and two monosaccharides (Man and Glc) would be used to identify Dioscorea opposita Thunb. polysaccharide (DOP) from different origins. The relationship of spectrum-effect showed that polysaccharides with features such as higher Mw1, a lower peak height of 1631.78 cm, higher content of Man, and lower content of Glc exerted stronger immune activity. In conclusion, this study established a polysaccharide-based quality evaluation method for Dioscorea opposita Thunb. and explored the relationship between polysaccharide fingerprints and in vitro immune activity, which provided a basis for further research on Dioscorea opposita Thunb.

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http://dx.doi.org/10.1016/j.ijbiomac.2024.135767DOI Listing

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