has been used extensively for research on the biology of aging due to its genetic tractability and short lifespan. In order to measure lifespan, populations of worms are synchronized so that all of the worms being measured begin the assay at the same age. This is typically accomplished by simply picking worms of a particular developmental stage to start the lifespan experiment or through bleaching, a process through which the body of the worm is dissolved in a solution of bleach (sodium hypochlorite) and sodium hydroxide to release its fertilized eggs. In this experiment, we examined the effect of bleaching on lifespan in wild-type worms and the long-lived mitochondrial mutant . We found that bleaching did not affect the longevity of wild-type worms or mutants. While we cannot exclude the possibility that bleaching affects the lifespan of specific genetic mutants, our results indicate that wild-type longevity is unaffected and that for at least some genetic mutants bleaching can be used for synchronization prior to initiating a lifespan experiment.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11409060 | PMC |
| http://dx.doi.org/10.17912/micropub.biology.001297 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Zinc speciation promotes distinct effects on dinoflagellate growth and coral trypsin-like enzyme activity.
Biometals
January 2025
Instituto de Química, Universidade de São Paulo, Av. Lineu Prestes 748, São Paulo, 05508-000, Brazil.
Zinc is an essential metal to living organisms, including corals and their symbiotic microalgae (Symbiodiniaceae). Both Zn(II) deprivation and overload are capable of leading to dysfunctional metabolism, coral bleaching, and even organism death. The present work investigated the effects of chemically defined Zn species (free Zn, ZnO nanoparticles, and the complexes Zn-histidinate and Zn-EDTA) over the growth of the dinoflagellates Symbiodinium microadriaticum, Breviolum minutum, and Effrenium voratum, and on the trypsin-like proteolytic activity of the hydrocoral Millepora alcicornis.
View Article and Find Full Text PDFPretrained Deep Neural Network Kin-SiM for Single-Molecule FRET Trace Idealization.
J Phys Chem B
January 2025
Single Molecule Analysis Group, Department of Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, United States.
Single-molecule fluorescence resonance energy transfer (smFRET) has emerged as a pivotal technique for probing biomolecular dynamics over time at nanometer scales. Quantitative analyses of smFRET time traces remain challenging due to confounding factors such as low signal-to-noise ratios, photophysical effects such as bleaching and blinking, and the complexity of modeling the underlying biomolecular states and kinetics. The dynamic distance information shaping the smFRET trace powerfully uncovers even transient conformational changes in single biomolecules both at or far from equilibrium, relying on trace idealization to identify specific interconverting states.
View Article and Find Full Text PDFFactors Affecting Chlorinated Product Formation from Sodium Hypochlorite Bleach and Limonene Reactions in the Gas Phase.
ACS EST Air
September 2024
Office of the Director, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, United States.
During use of sodium hypochlorite bleach, gas-phase hypochlorous acid (HOCl) and chlorine (Cl) are released, which can react with organic compounds present in indoor air. Reactivity between HOCl/Cl and limonene, a common constituent of indoor air, has been observed. The purpose of this study was to characterize the chemical species generated from gas-phase reactions between HOCl/Cl and limonene.
View Article and Find Full Text PDFHardness, roughness, and compressive strength of different filled resin composites after thermal aging and bleaching procedures.
Am J Dent
December 2024
Department of Prosthodontics, Faculty of Dentistry, Bursa Uludağ University, Bursa, Turkey.
Purpose: To evaluate the effect of aging and bleaching on the physico-mechanical properties of the different filled resin composites.
Methods: Samples of resin composites [Arabesk (A), G-aenial Anterior (G), i-LIGHT N (I) and Palfique Estelite Paste (P) \ were first divided into two subgroups. Thermal aging was applied to half of the samples.
Complexity in the Photofunctionalization of Single-Wall Carbon Nanotubes with Hypochlorite.
ACS Nano
January 2025
Department of Chemistry and the Smalley-Curl Institute, Rice University, Houston, Texas 77005, United States.
The reaction of aqueous suspensions of single-wall carbon nanotubes (SWCNTs) with UV-excited sodium hypochlorite has previously been reported to be an efficient route for doping nanotubes with oxygen atoms. We have investigated how this reaction system is affected by pH level, dissolved O content, and radical scavengers and traps. Products were characterized with near-IR fluorescence, Raman, and XPS spectroscopy.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!