Mechanistic study of leukopenia treatment by Qijiao shengbai Capsule via the Bcl2/Bax/CASAPSE3 pathway.

Front Pharmacol

State Key Laboratory of Functions and Applications of Medicinal Plants and School of Pharmacy, Guizhou Medical University, Guiyang, China.

Published: September 2024

AI Article Synopsis

  • Chemotherapy can lead to leukopenia by suppressing bone marrow function and effectiveness in cancer treatments; Qijiao Shengbai Capsule (QJSB) is commonly used to manage this condition but its active components are not fully understood.
  • This study utilized network pharmacology and molecular docking to analyze QJSB’s ingredients and mechanisms, identifying 16 key components that increase white blood cell counts in mice with leukopenia.
  • The findings suggest that QJSB influences cell growth related genes and prevents apoptosis through specific signaling pathways, potentially enhancing immune function by boosting CD4 T cell levels.

Article Abstract

Background: Leukopenia can be caused by chemotherapy, which suppresses bone marrow function and can impact the effectiveness of cancer treatment. Qijiao Shengbai Capsule (QJSB) is commonly used to treat leukopenia, but the specific bioactive components and mechanisms of action are not well understood.

Objectives And Results: This study aimed to analyze the active ingredients of QJSB and its potential targets for treating leukopenia using network pharmacology and molecular docking. Through a combination of serum pharmacochemistry, multi-omics, network pharmacology, and validation experiments in a murine leukopenia model, the researchers sought to understand how QJSB improves leukopenia. The study identified 16 key components of QJSB that act to increase the number of white blood cells in leukopenic mice. Multi-omics analysis and network pharmacology revealed that the PI3K-Akt and MAPK signaling pathways are important in the treatment of leukopenia with QJSB. Five specific targets (JUN, FOS, BCl-2, CASPAS-3) were identified as key targets.

Conclusion: Validation experiments confirmed that QJSB regulates genes related to cell growth and inhibits apoptosis, suggesting that apoptosis may play a crucial role in leukopenia development and that QJSB may improve immune function by regulating apoptotic proteins and increasing CD4 T cell count in leukopenic mice.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11408280PMC
http://dx.doi.org/10.3389/fphar.2024.1451553DOI Listing

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