() is bovine mastitis pathogen and has thus became a matter of concern to dairy farms worldwide in terms of economic loss. The aims of this study were to (a) determine virulence genes, and (b) characterize the antimicrobial resistance (AMR) profiles and AMR genes and (c) figure out the relationship between AMR phenotypes and genotypes of isolated from dairy cows in north China. A total of 20 virulence genes and 23 AMR genes of 140 isolates collected from 12 farms in six provinces were studied. The antimicrobial susceptibility of 10 veterinary commonly used antimicrobials were tested using the broth microdilution method. Results showed that all the isolates harbored the virulence genes IV, , and . The isolates that harbored the genes , , , and exhibited the high prevalence (99.29%), followed by isolates that harbored I (98.57%), (97.86%), (97.14%), II (92.14%), (52.14%), (25%), (2.14%), and (0.71%). The , , , , and genes were not detected. The virulence patterns of B (___ ___ __) and C (__ _ _ _) were dominant, accounting for 97.86% of the isolates. The following AMR genes were prevalent: (97.14%), (M) (95.00%), (A) (80.71%), (B) (75.00%), (O) (72.14%), (49.29%), (S) (29.29%), (25.71%), (A) (17.14%), (C) (13.57%), (L) (10.71%), (2.86%), and (TR) (2.86%). The , , , (D), (F/G/Q), and (A) genes were not detected. Eighty percent of the isolates harbored AMR genes and were highly resistant to tetracycline, followed by macrolides (10.71%), lincosamides (9.29%) and β-lactams (4.29%). In conclusion, isolates only exhibited well correlation between tetracyclines resistance phenotype and genotype, and almost all isolates harbored intact combination of virulence genes.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11409094 | PMC |
| http://dx.doi.org/10.3389/fcimb.2024.1417299 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilis.
Ann Clin Microbiol Antimicrob
January 2025
Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
Proteus mirabilis (P. mirabilis) is one of the most important causative pathogens associated with complicated urinary tract infections with a 20% incidence. For epidemiological determinations, several phenotypic and molecular typing methods have been implicated.
View Article and Find Full Text PDFThe Pathogenicity Traits of Avian Pathogenic E. coli (APEC) O25-ST131 Associated with Avian Colibacillosis in Georgia Poultry and their Genotypic and Phenotypic Overlap with other ExPEC.
J Appl Microbiol
January 2025
Department of Population Health, College of Veterinary Medicine, 501 D.W. Brooks Dr., University of Georgia, Athens, GA 30602.
Aims: To characterize Escherichia coli O25 ST131 (O25-ST131) isolated from Georgia poultry, - a "global high-risk" clonal strain.
Methods And Results: Using multiplex PCR to detect target genes in 98 isolates of avian pathogenic E. coli (APEC) O25 recovered from avians diagnosed with colibacillosis (n=87) and healthy chicks (n=11) in Georgia, USA.
Decarboxylase mediated oxalic acid metabolism is important to antioxidation and detoxification rather than pathogenicity in .
Virulence
January 2025
The Key Laboratory for Extreme-Environmental Microbiology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Oxalic acid (OA), an essential pathogenic factor, has been identified in several plant pathogens, and researchers are currently pursuing studies on interference with OA metabolism as a treatment for related diseases. However, the metabolic route in remains unknown. In this study, we describe D-erythroascorbic acid-mediated OA synthesis and its metabolic and clearance pathways in rice blast fungus.
View Article and Find Full Text PDFWhole-exome sequencing identifies novel mutation in intrahepatic cholestasis of pregnancy: A case report and literature review.
Z Geburtshilfe Neonatol
January 2025
Department of Obstetrics and Gynecology, Sichuan University West China Second University Hospital, Chengdu, China.
Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific liver disease characterized by pruritus and elevated total bile acid (TBA) levels. The most serious impact of ICP is sudden unexplained intrauterine fetal death, especially when an associated TBA ≥ 100 µmol/L is confirmed.We report a case of a 27-year-old female patient with early-onset severe refractory ICP.
View Article and Find Full Text PDFVirulence expression difference to intestinal cells of different pathogenic Listeria monocytogenes contaminating sausages after simulated digestive tract.
Int J Food Microbiol
January 2025
State Key Laboratory of Meat Quality Control and Cultured Meat Development, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, PR China. Electronic address:
This study investigated the difference in survival among Listeria monocytogenes (LM) 10403S (highly pathogenic strain) and M7 (low pathogenic strain) in sausage under a simulated digestive environment, and established intestinal organoids and macrophages co-culture model to further explore the virulence expression difference to intestinal cells between LM 10403S and M7 after in vitro gastrointestinal digestion. Results showed that, compared with LM M7, LM 10403S exhibited a high survival rate during in vitro digestion, which may be due to the increased expression of stress response-related genes. In addition, the expression of virulence genes in LM 10403S was significantly higher than in LM M7 under the gastrointestinal environment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!