Synthesis of Indolyl Isothiocyanate and Its Inhibitory Activity against Fungi.

Based on previous research, this study synthesized 24 compounds by splicing the substructures of the indolyl group and the isothiocyanate group. , , and were used to test the activity of the target compounds. At 100 μg/mL, compounds , , , and exhibited excellent inhibitory effects of more than 80% on , and . The EC values of compounds and were 0.64 and 2.08 μg/mL, respectively. Potted antifungal activity demonstrated that compounds and had a protective effect of around 80% against at 200 μg/mL. Further physiological and biochemical studies on revealed that compound 13 thickened cell walls and caused mitochondrial vacuolization. Moreover, theoretical calculations indicated that the charge distribution of indolyl isothiocyanate compounds played a crucial role in the observed fungicidal activity. In summary, this study provided fundamental reference data for the derivative synthesis of these indolyl isothiocyanate compounds.