Obtaining reliable and informative DNA data from soil samples is challenging due to the presence of interfering substances and typically low DNA yields. In this work, we prepared poly(ethylene glycol)-modified magnetic particles (PEG@FeO) for DNA purification. The particles leverage the facilitative effect of calcium ions (Ca), which act as bridges between DNA and PEG@FeO by coordinating with the phosphate groups of DNA and the hydroxyl groups on the particles. The addition of 2-propanol further enhances this Ca-mediated DNA adsorption by inducing a conformational change from the B-form to the more compact A-form of DNA. PEG@FeO demonstrates a DNA adsorption capacity of 144.6 mg g. When applied to the extraction of genomic DNA from soil samples, PEG@FeO outperforms commercial kits and traditional phenol-chloroform extraction methods in terms of DNA yield and purity. Furthermore, we developed a 16-channel automated DNA extraction device to streamline the process and reduce the extraction time. The successful amplification of target bacterial and fungal amplicons underscores the potential of this automated, device-assisted method for studying soil microbial diversity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.langmuir.4c02198 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Four SNPs in the untranslated region of the NOTCH2 gene involved in reproductive traits in fertile and anestrum Holstein Friesian cattle.
BMC Vet Res
December 2024
Department of Research, Research and Development Station for Bovine, Arad, Romania.
Background: There are no studies belong NOTCH2 gene polymorphism in relation to reproductive and productive traits in Holstein cattle. The objective of the present study was to investigate the effect of NOTCH2 gene polymorphisms on productive and reproductive performance of fertile and anestrum cattle.
Methods: The cattle were classified into anestrus for 3-12 months postpartum (n = 115, 37.
Ehrlichia sp. in dairy cattle from Bahia, Brazil: high seropositivity rates and molecular confirmation of Ehrlichia minasensis.
BMC Vet Res
December 2024
Departamento de Anatomia, Patologia e Clínicas Veterinárias, Universidade Federal da Bahia, Escola de Medicina Veterinária e Zootecnia, Av. Milton Santos 500, Salvador, Bahia, CEP 40170-110, Brazil.
Background: Ehrlichia spp. are obligate intracytoplasmic Gram-negative tickborne bacteria from the Anaplasmataceae family. Ehrlichiosis is considered an emerging disease in humans and animals.
View Article and Find Full Text PDFThe transcription factor YbdO attenuates the pathogenicity of avian pathogenic Escherichia coli by regulating oxidative stress response.
BMC Microbiol
December 2024
College of Agriculture and Forestry, Linyi University, Linyi, 276005, Shandong, China.
Avian pathogenic Escherichia coli (APEC) is a significant pathogen infecting poultry that is responsible for high mortality, morbidity and severe economic losses to the poultry industry globally, posing a substantial risk to the health of poultry. APEC encounters reactive oxygen species (ROS) during the infection process and thus has evolved antioxidant defense mechanisms to protect against oxidative damage. The imbalance of ROS production and antioxidant defenses is known as oxidative stress, which results in oxidative damage to proteins, lipids and DNA, and even bacterial cell death.
View Article and Find Full Text PDF"High specificity of PCR in diagnosing mucocutaneous leshminiasis: a systematic review and meta analysis".
BMC Infect Dis
December 2024
Department of Community Health Sciences, Aga Khan University, Karachi, Pakistan.
Background: Mucocutaneous leishmaniasis (MCL) is a severe form of leishmaniasis causing chronic and destructive lesions. Accurate diagnosis is crucial for effective treatment. Traditional methods, such as the Montenegro skin test is delayed hypersensitivity test.
View Article and Find Full Text PDFAntimicrobial resistance, virulence gene profiling, and genetic diversity of multidrug-resistant Pseudomonas aeruginosa isolates in Mazandaran, Iran.
BMC Microbiol
December 2024
Department of Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Background: Pseudomonas aeruginosa is a major cause of healthcare-associated infections (HAIs), particularly in immunocompromised patients, leading to high morbidity and mortality rates. This study aimed to investigate the antimicrobial resistance patterns, virulence gene profiles, and genetic diversity among P. aeruginosa isolates from hospitalized patients in Mazandaran, Iran.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!