AI Article Synopsis
- Alcohol withdrawal affects social behavior differently in male and female rodents, which can be measured using the three-chamber test; however, traditional automatic tracking methods often yield inaccurate results due to their inability to capture direct interactions.* -
- The study utilized DeepLabCut, an AI-based pose-estimation toolkit, to score social behavior in mice during alcohol withdrawal and demonstrated that it could detect significant social deficits in female mice, contrary to results from conventional automatic tracking methods like AnyMaze.* -
- Results indicated that DeepLabCut and manual scoring methods showed strong agreement and lower biases in analyzing social deficits during acute withdrawal, suggesting that this AI approach provides a more accurate assessment of social behavior compared to existing commercial tracking systems.*
Article Abstract
Background: Alcohol consumption and withdrawal alter social behaviour in humans in a sex-dependent manner. The three-chamber test is a widely used paradigm to assess rodents' social behaviour, including sociability and social novelty. Automatic tracking systems are commonly used to score time spent with conspecifics, despite failing to score direct interaction time with conspecifics rather than time in the nearby zone. Thereby, the automatically scored results are usually inaccurate and need manual corrections.
New Method: New advances in artificial intelligence (AI) have been used recently to analyze complex behaviours. DeepLabCat is a pose-estimation toolkit that allows the tracking of animal body parts. Thus, we used DeepLabCut, to introduce a scoring model of the three-chamber test to investigate alcohol withdrawal effects on social behaviour in mice considering sex and withdrawal periods. We have compared the results of two automatic pose estimation methods: automatic tracking (AnyMaze) and DeepLabCut considering the manual scoring method, the current gold standard.
Results: We have found that the automatic tracking method (AnyMaze) has failed to detect the significance of social deficits in female mice during acute withdrawal. However, tracking the animal's nose using DeepLabCut showed a significant social deficit in agreement with manual scoring. Interestingly, this social deficit was shown only in females during acute and recovered by the protracted withdrawal. DLC and manually scored results showed a higher Spearman correlation coefficient and a lower bias in the Bland-Altman analysis.
Conclusion: our approach helps improve the accuracy of scoring the three-chamber test while outperforming commercial automatic tracking systems.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11403434 | PMC |
| http://dx.doi.org/10.1016/j.heliyon.2024.e36352 | DOI Listing |
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