AI Article Synopsis

  • Three-dimensional correlative multimodal and multiscale imaging is a new technique designed to study complex biological materials like bone by combining different images across various scales for a fuller understanding of their structure.
  • Researchers created a specific workflow for analyzing human trabecular bone, using a femtosecond laser to create precise reference grids that help correlate images and identify features like bone cells in high detail.
  • This advanced imaging method offers the potential for detailed structural analysis not only of bone but also of other biological materials and engineered substances, providing valuable insights into their architecture from large to tiny scales.

Article Abstract

Three-dimensional correlative multimodal and multiscale imaging is an emerging method for investigating the complex hierarchical structure of biological materials such as bone. This approach synthesizes images acquired across multiple length scales, for the same region of interest, to provide a comprehensive view of the material structure of a sample. Here, we develop a workflow for the structural analysis of human trabecular bone using a femtosecond laser to produce a precise grid to facilitate correlation between imaging modalities and identification of structures of interest, in this case, a single trabecula within a volume of trabecular bone. Through such image registration, high resolution X-ray microscopy imaging revealed fine architectural details, including the cement sheath and bone cell lacunae of the selected bone trabecula. The selected bone volume was exposed with a combination of manual polishing and site-specific femtosecond laser ablation and then examined with plasma focused ion beam-scanning electron microscopy. This reliable and versatile correlation approach has the potential to be applied to a variety of biological tissues and traditional engineered materials. The proposed workflow has the enhanced capability for generating highly resolved and broadly contextualized structural data for a better understanding of the architectural features of a material spanning its macroscopic to nanoscopic levels.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11405866PMC
http://dx.doi.org/10.1038/s41598-024-72739-8DOI Listing

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