AI Article Synopsis
- A study investigates antibacterial and antivirulence compounds derived from bacterial metabolites, specifically focusing on enteropathogenic E. coli (EPEC), a major cause of intestinal infections in infants.
- Researchers examined marine actinobacteria from the Arctic Sea for their ability to inhibit EPEC's harmful effects on intestinal cells without affecting bacterial growth.
- Key discoveries include two promising compounds: one that interferes with EPEC's ability to alter actin polymerization and another that inhibits EPEC growth, highlighting the potential of marine bacteria as sources of new antimicrobial agents.
Article Abstract
A considerable number of antibacterial agents are derived from bacterial metabolites. Similarly, numerous known compounds that impede bacterial virulence stem from bacterial metabolites. Enteropathogenic (EPEC) is a notable human pathogen causing intestinal infections, particularly affecting infant mortality in developing regions. These infections are characterized by microvilli effacement and intestinal epithelial lesions linked with aberrant actin polymerization. This study aimed to identify potential antivirulence compounds for EPEC infections among bacterial metabolites harvested from marine actinobacteria ( sp. and spp.) from the Arctic Sea by the application of virulence-based screening assays. Moreover, we demonstrate the suitability of these antivirulence assays to screen actinobacteria extract fractions for the bioassay-guided identification of metabolites. We discovered a compound in the fifth fraction of a strain that interferes with EPEC-induced actin polymerization without affecting growth. Furthermore, a growth-inhibiting compound was identified in the fifth fraction of a strain. Our findings include the bioassay-guided identification, HPLC-MS-based dereplication, and isolation of a large phospholipid and a likely antimicrobial peptide, demonstrating the usefulness of this approach in screening for compounds capable of inhibiting EPEC virulence.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11392781 | PMC |
| http://dx.doi.org/10.3389/fmicb.2024.1432475 | DOI Listing |
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