A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 143

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 994
Function: getPubMedXML

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3134
Function: GetPubMedArticleOutput_2016

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Impact of swabbing solutions on the recovery of biological material from non-porous surfaces. | LitMetric

Impact of swabbing solutions on the recovery of biological material from non-porous surfaces.

Forensic Sci Int Synerg

Leverhulme Research Centre for Forensic Science, Fleming Gym Building, University of Dundee, Small's Wynd, Dundee, United Kingdom.

Published: August 2024

AI Article Synopsis

  • Cotton swabs are crucial for collecting biological evidence, but their effectiveness in retrieving DNA is influenced by factors like technique, DNA source, and moisteners used.
  • The study explored various swab-moistening solutions such as buffers, detergents, and chelators to assess their impact on DNA recovery from different surfaces.
  • Findings revealed that chelating agents (EGTA and EDTA) excelled at recovering DNA from blood and saliva, while water and detergents were better for collecting cell-free and cellular DNA from trace deposits.

Article Abstract

Cotton swabs are one of the most effective methods of retrieving biological evidence. The efficiency of swab-based DNA recovery is impacted by many factors, such as the swabbing technique, source of DNA and volume and type of wetting solution used to moisten the swab head. This study aimed to evaluate a series of different swab-moistening solutions. The types of swabbing solutions included buffers, detergent-based solutions, and chelating agents. The DNA deposits, including cell-free DNA, cellular DNA, blood, and saliva, were collected from three non-porous surfaces: plastic, glass, and metal. The difference in the performance of the swab-wetting solutions was heavily influenced by the type of biological fluid, with the chelating agents, EGTA and EDTA, being the most suitable for recovering DNA from saliva and blood samples. Conversely, water and detergent-based solutions were more appropriate for cell-free and cellular DNA material likely to be found in trace DNA deposits.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11399651PMC
http://dx.doi.org/10.1016/j.fsisyn.2024.100551DOI Listing

Publication Analysis

Top Keywords

swabbing solutions
8
non-porous surfaces
8
dna
8
detergent-based solutions
8
chelating agents
8
dna deposits
8
cellular dna
8
solutions
6
impact swabbing
4
solutions recovery
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: