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Imagerie time-lapse et intelligence artificielle : Ce n'est que la fin du début!
J Obstet Gynaecol Can
September 2024
Department of Obstetrics and Gynecology, Baylor Scott & White, Temple, TX; Department of Reproductive Medicine, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:
Vitrification of Human Oocytes Before or After Rescue-IVM Does not Impair Maturation Kinetics but Induces Meiotic Spindle Alterations.
Reprod Sci
September 2024
Université Clermont Auvergne, Inserm, Imagerie Moléculaire Et Stratégies Théranostiques, UMR 1240, Clermont-Ferrand, France.
Cryopreservation of in vitro matured oocytes is still considered as an experimental alternative to mature oocyte vitrification after ovarian stimulation. Here, we investigated whether rescue-IVM should be performed before or after vitrification. For this, 101 immature oocytes (germinal vesicle stage) from women undergoing ICSI were used.
View Article and Find Full Text PDFLysate-based pipeline to characterize microtubule-associated proteins uncovers unique microtubule behaviours.
Nat Cell Biol
February 2022
Institut Curie, Université PSL, CNRS UMR3348, Orsay, France.
The microtubule cytoskeleton forms complex macromolecular assemblies with a range of microtubule-associated proteins (MAPs) that have fundamental roles in cell architecture, division and motility. Determining how an individual MAP modulates microtubule behaviour is an important step in understanding the physiological roles of various microtubule assemblies. To characterize how MAPs control microtubule properties and functions, we developed an approach allowing for medium-throughput analyses of MAPs in cell-free conditions using lysates of mammalian cells.
View Article and Find Full Text PDFMicrovascular maturation by mesenchymal stem cells in vitro improves blood perfusion in implanted tissue constructs.
Biomaterials
January 2021
Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS UMR7241, INSERM U1050, PSL Research University, Paris, France. Electronic address:
Blood perfusion of grafted tissue constructs is a hindrance to the success of stem cell-based therapies by limiting cell survival and tissue regeneration. Implantation of a pre-vascularized network engineered in vitro has thus emerged as a promising strategy for promoting blood supply deep into the construct, relying on inosculation with the host vasculature. We aimed to fabricate in vitro tissue constructs with mature microvascular networks, displaying perivascular recruitment and basement membrane, taking advantage of the angiogenic properties of dental pulp stem cells and self-assembly of endothelial cells into capillaries.
View Article and Find Full Text PDFBiofilm-coated microbeads and the mouse ear skin: An innovative model for analysing anti-biofilm immune response in vivo.
PLoS One
January 2021
Laboratoire Microorganismes: Génome et Environnement, Université Clermont Auvergne, UMR CNRS 6023, Clermont-Ferrand, France.
Owing to its ability to form biofilms, Staphylococcus aureus is responsible for an increasing number of infections on implantable medical devices. The aim of this study was to develop a mouse model using microbeads coated with S. aureus biofilm to simulate such infections and to analyse the dynamics of anti-biofilm inflammatory responses by intravital imaging.
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