AI Article Synopsis
- Fusarioid fungi, like Neocosmospora solani and Fusarium oxysporum, are becoming important human pathogens causing a range of infections, which makes accurate identification and preservation in labs crucial.
- This study introduced a new medium called milk-honey and malt agar (MHM) that outperformed traditional media (like Sabouraud dextrose agar) in cultivating and preserving fusarioid fungi isolates, significantly enhancing conidia production and viability.
- MHM showed distinct growth patterns for different fusarioid species, highlighting its potential as an effective tool for clinical mycology laboratories, especially in resource-limited environments.
Article Abstract
Fusarioid fungi, particularly Neocosmospora solani and Fusarium oxysporum, are emerging as significant human pathogens, causing infections ranging from localized mycoses to life-threatening systemic diseases. Accurate identification and preservation of these fungi in clinical laboratories remain challenging because of their diverse morphologies and specific growth requirements. This study evaluated a novel milk-honey and malt agar (MHM) against conventional media for cultivating and preserving 60 clinical fusarioid isolates, including Neocosmospora spp. (n = 47), Bisifusarium spp. (n = 5), and Fusarium spp. (n = 8). Compared with Sabouraud dextrose 2 % agar (SDA) and malt extract agar (ME2), MHM significantly increased conidia production (p < 0.0001, mean = 3.4 × 10, standard deviation (SD) = ±1.3 × 10), with results similar to those of carnation leaf agar (CLA). MHM facilitated superior preservation of fusarioid viability for up to one year at room temperature on slant cultures and over two years on swabs in Amies gel with charcoal, outperforming current methods such as Castellani (water) or cryopreservation. Morphological characterization of fusarioid fungi grown on MHM revealed distinct growth patterns and conidial structures for Neocosmospora, Bisifusarium, and Fusarium species, aiding in identifying these genera. The superior performance of MHM in stimulating conidiation, maintaining viability, and preserving morphology underscore its potential as a reference medium for medically relevant fusarioid fungi, with broad implications for clinical mycology laboratories and resource-limited settings.
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| http://dx.doi.org/10.1016/j.mimet.2024.107042 | DOI Listing |
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