Spectrophotometric determination of o-phenylphenol in canned drinks using three-phase hollow-fiber liquid phase microextraction.

A three-phase hollow-fiber liquid phase microextraction for o-phenylphenol (OPP) determination was developed. 1-octanol was employed as the organic phase, impregnated within the pores of the hollow fiber wall which was immersed in the sample solution, serving as a donor phase. OPP in the sample solution was extracted via octanol in the fiber pores into NaOH, which acted as the acceptor phase in the lumen of the fiber. The extracted OPP was then subjected to spectrophotometric detection at 712 nm using the indophenol blue reaction. The developed method showed a linear calibration curve (0.002-0.040 mg L) with high sensitivity (5.75 L mg), low limit of detection (0.31 μg L), and high recovery (73.6-94.8 %). Intra-day and inter-day precision at 2.1 μg L OPP were 7.4 % (n = 12) and 10.9 % (n = 4) relative standard deviations, respectively. The determined OPP in various canned drinks was found to be between 2.0 and 17.8 μg L using the developed method.