Adventitious root (AR) culture of Atractylodes chinensis is an efficient platform for sustainable production of its sesquiterpenoid compounds (atractylon and β-eudesmol). However, their limited accumulation levels need an effective elicitation approach, and the present study solved this problem using methyl jasmonate (MeJA) as an elicitor. The effects of its treatment concentration and duration on metabolite production were investigated. The ARs treated with 100 µM MeJA for 7 d increased atractylon and β-eudesmol by 3.64- and 1.90-fold, respectively, compared with the control. We further performed transcriptome analysis to explore the transcriptional regulation mechanism of the MeJA elicitation. A total of 124,464 unigenes were identified in A. chinensis ARs, of which 3,568 genes were upregulated and 3,864 genes were downregulated with the MeJA treatment. The MeJA treatment activated the endogenous jasmonic acid biosynthesis and signaling pathways and sesquiterpenoid biosynthesis. In addition, MeJA treatment more significantly activated genes of the methylerythritol phosphate pathway than of the mevalonate pathway. In addition, 14 genes encoding terpene synthase were identified to be significantly upregulated. A total of 2,700 transcription factors (TFs) were identified in A. chinensis ARs, of which Tify, MYB and MADS were significantly enriched under the MeJA treatment. We predicted a new antagonistic interaction between MYC2 and CPP TFs, which was significantly regulated by the MeJA treatment. The results of real-time quantitative PCR and enzyme activity assays proved the reliability of the transcriptome data. This study will help improve the in vitro production system of A. chinensis sesquiterpenoids and understand the transcriptional regulation mechanism of MeJA elicitation.
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http://dx.doi.org/10.1093/pcp/pcae104 | DOI Listing |
BMC Plant Biol
December 2024
Faculty of Agriculture, Department of Horticulture, Ordu University, Ordu, Türkiye.
Background: Owing to its high perishability, the market life of nectarine fruit is very short. Cold storage is a principal approach to limit post-harvest quality loss in nectarines. The objective of this research was to evaluate the impact of postharvest methyl jasmonate (MeJA), salicylic acid (SA) and 1-methylcyclopropene (1-MCP) on quality properties of nectarine fruit, specifically weight loss, firmness, phenolics and antioxidant activity, following cold storage and subsequent shelf life.
View Article and Find Full Text PDFZhongguo Zhong Yao Za Zhi
October 2024
Analytical and Testing Center, Anhui Dabieshan Chinese Medicine Research Institute, Anhui Engineering Research Center for Eco-agriculture of Traditional Chinese Medicine, West Anhui University Lu'an 237012, China.
UDP-rhamnose, which is synthesized under the catalysis by the rhamnose synthase(RHM), is an essential sugar donor for the synthesis of rhamnoside in plants. Based on the reported rhamnose synthase, this study screened one RHM gene(DhuRHM) from the localized gene database of Dendrobium huoshanense by sequence alignment. This gene was cloned, and then bioinformatics analysis and in vitro functional verification were carried out.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
In the process of catalyzing carotenoids into various apocarotenoids and other derivatives, carotenoid cleavage dioxygenases (CCDs) play key roles. However, little information on CCDs has been reported in regard to . In this study, a total of 21 CCD genes were identified in the whole genome of , mainly distributed between five chromosomes.
View Article and Find Full Text PDFInt J Mol Sci
November 2024
College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Plants (Basel)
November 2024
Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang 50463, Republic of Korea.
Brown spot (BS) is caused by necrotrophs fungi () which affects rainfed and upland production in rice, resulting in significant losses in yield and grain quality. Here, we explored the meJA treatment that leads to rice resistance to BS. Fibrillins (FBNs) family are constituents of plastoglobules in chloroplast response to biotic and abiotic stress, many research revealed that and are not only associated with the rice against disease but also with the JA pathway.
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