Conservation and Divergence of Gene Family in Different Ploidy Bamboos.

Phosphoenolpyruvate carboxylase (PEPC), as a necessary enzyme for higher plants to participate in photosynthesis, plays a key role in photosynthetic carbon metabolism and the stress response. However, the molecular biology of the family of Bambusoideae has been poorly studied, and the function of its members in the growth and development of Bambusoideae is still unclear. Here, we identified a total of 62 PEPC family members in bamboo. All the genes in the bamboo subfamily were divided into twelve groups, each group typically containing significantly fewer members in than in , and . The results of an intraspecific and interspecies collinearity analysis showed that fragment replication and whole genome replication were the main driving forces of bamboo members. Furthermore, the / values of collinear genes showed that bamboo experienced purification selection. In addition, the promoter region of genes contains cis-acting elements related to light response, plant hormone response and response to stress. An analysis of the expression levels of the family in different developmental stages and tissues of bamboo shoots has shown that , and were highly expressed in the leaves of non-flowering plants and culms. Furthermore, was significantly upregulated in leaves after GA treatment. Further research has shown that was mainly localized in the cell membrane. This provides a solid bioinformatics foundation for further understanding the biological functions of the bamboo family.