AI Article Synopsis
- * Self-splicing ribozymes are a key method for generating circRNAs, known for their simplicity and efficiency in circularizing target genes.
- * The review discusses recent advancements in self-circularization techniques using self-splicing ribozymes, including group I and II intron ribozymes, along with their advantages and disadvantages for circRNA preparation.
Article Abstract
In vitro circular RNA (circRNA) preparation methods have been gaining a lot of attention recently as several reports suggest that circRNAs are more stable, with better performances in cells and in vivo, than linear RNAs in various biomedical applications. Self-splicing ribozymes are considered a major in vitro circRNA generation method for biomedical applications due to their simplicity and efficiency in the circularization of the gene of interest. This review summarizes, updates, and discusses the recently developed self-circularization methods based on the self-splicing ribozyme, such as group I and II intron ribozymes, and the pros and cons of each method in preparing circRNA in vitro.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11394858 | PMC |
| http://dx.doi.org/10.3390/ijms25179437 | DOI Listing |
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