The aim was to assess the impact of long-term storage on the quality of Botucatu rabbit meat frozen in pre- and post-rigor stages. The stability of the technological and nutritional parameters of (LL) muscle was analyzed over 12 months. In the post-rigor phase, the dorsal LL surface showed a higher level ( < 0.05) of redness and saturation, while the ventral surface showed a higher level ( < 0.05) of yellowness compared to the pre-rigor LL muscle. During storage, the redness and saturation in the LL muscle decreased ( < 0.05), while the yellowness increased ( < 0.05) on both dorsal and ventral surfaces. In the first six months, the pre-rigor meat had a higher pH ( < 0.05) compared to the post-rigor meat. The fresh meat showed higher ( < 0.05) shear force values in the post-rigor stage. Over the 12-month study period, the lipid oxidation, myofibrillar fragmentation index, gross energy, and levels of protein, fat, and carbohydrates increased ( < 0.05), while the shear force, mineral content, and moisture decreased ( < 0.05). Thus, rigor mortis affects meat color in Botucatu rabbits. Fresh meat in the pre-rigor stage is softer, moister, and less acidic than post-rigor meat after 24 h of chilling. Long-term freezing enhances tenderness, regardless of the rigor phase at freezing, preserving its physical, chemical, and nutritional quality, with minor changes in color, lipid oxidation, and chemical composition.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11394318PMC
http://dx.doi.org/10.3390/ani14172510DOI Listing

Publication Analysis

Top Keywords

long-term freezing
8
pre- post-rigor
8
technological nutritional
8
nutritional parameters
8
parameters muscle
8
botucatu rabbits
8
surface higher
8
higher level
8
005
8
level 005
8

Similar Publications

Eutectogels are recently emerged as promising alternatives to hydrogels owing to their good environmental stability derived from deep eutectic solvents (DES). However, construction of competent eutectogels with both high conductivity and mechanical toughness is still difficult to achieve yet highly demanded. In this work, new LMNP-PEDOT-CMC-AA (LPCA) eutectogels are prepared using acrylic acid (AA) and carboxymethylcellulose sodium (CMC) as polymeric networks, liquid metal nanoparticle-poly(3,4-ethylenedioxythiophene) (LMNP-PEDOT) are added as multifunctional soft fillers.

View Article and Find Full Text PDF

Bexicaserin is a highly selective agonist at the 5-HT receptor in clinical development for the treatment of seizures associated with developmental and epileptic encephalopathies (DEEs). We report an LC-MS/MS method for the quantitative estimation of bexicaserin in human plasma and urine. The sample preparation involves the extraction of bexicaserin and internal standard (CD-bexicaserin; IS) from 150 μL plasma and 50 μL urine using a solid phase extraction method.

View Article and Find Full Text PDF

Here, we report a simple method to prepare near-IR (NIR) surface-enhanced Raman scattering (SERS) substrates by quickly freezing a citrate-capped Au nanoparticle (AuNP) solution in liquid nitrogen, followed by thawing it at room temperature. This process aggregates AuNPs in a controlled manner by forming ice crystals with smaller grain sizes when compared to a slow freezing process. The resulting smaller AuNP aggregates remain suspended in solution long enough to conduct high-throughput chemical analysis in a microwell plate using the NIR SERS spectroscopy.

View Article and Find Full Text PDF

Extracellular vesicles (EVs) are widely investigated for their implications in cell-cell signaling, immune modulation, disease pathogenesis, cancer, regenerative medicine, and as a potential drug delivery vector. However, maintaining integrity and bioactivity of EVs between Good Manufacturing Practice separation/filtration and end-user application remains a consistent bottleneck towards commercialization. Milk-derived extracellular vesicles (mEVs), separated from bovine milk, could provide a relatively low-cost, scalable platform for large-scale mEV production; however, the reliance on cold supply chain for storage remains a logistical and financial burden for biologics that are unstable at room temperature.

View Article and Find Full Text PDF

The pharmaceutical quality of freeze-dried tablets containing therapeutic bacteriophages against Pseudomonas aeruginosa and Staphylococcus aureus.

Int J Pharm

January 2025

Department of Experimental Biology, Division of Genetics and Molecular Biology, Faculty of Science, Masaryk University, 611 37 Brno, Czech Republic. Electronic address:

The preparation of a solid dosage form containing bacteriophages, which meets pharmaceutical requirements and ensures long-term stability of the phage effect, is significant for implementing phage therapy in practice. A commonly used method for processing phages into a solid form is freeze-drying into a (so-called) freeze-dried cake; however, to date there have been no studies examining the pharmacopeial parameters of freeze-dried tablets with bacteriophages. In this study, we describe the preparation and properties of freeze-dried tablets containing a cocktail of purified pseudomonal bacteriophage DSM 33593 from the genus Pbunavirus and staphylococcal bacteriophage DSM 33473 from the genus Kayvirus (10 PFU/tablet) as the active ingredient.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!