Pesticide residues and risk assessment of trunk-injected pesticides in pine nut (Pinus koraiensis) seeds.

Pine nuts, the edible seeds of pines (Family: Pinaceae, Pinus spp.), are popular worldwide, particularly those from the Korean pine tree (Pinus koraiensis), which is economically significant and widely exported. The spread of pine wilt disease (PWD) caused by pinewood nematodes (PWNs) has necessitated the use of trunk injections of pesticides in Korea, raising concerns about pesticide residues in edible pine nuts. This study investigated the concentration of pesticide residues in pine nuts from trunk-injected trees and assessed their potential health risks to consumers. After Korean pine tree was trunk-injected with pesticide, the pine nuts were subsequently harvested and analyzed for pesticide residues using LC‒MS/MS. Short- and long-term risk assessments of trunk-injected pesticides in pine nuts were conducted. Abamectin and emamectin benzoate were not detected, while acetamiprid and sulfoxaflor were detected, but their levels were below the maximum residue limits (MRLs) set by Korea, the EU, and the US. The short-term and long-term risk assessments indicated negligible health risks for consumers from these residues. Despite the low risk, we recommend continued monitoring of pesticide residues in pine nuts intended for sale to ensure consumer safety.