Utilization of duckweed-derived biomass for polyhydroxybutyrate production in Escherichia coli via dual enzymatic saccharification using amylase and cellulase complex.

Duckweed is a rapid-growing plant with a high starch and low lignin content. The duckweed was saccharified via dual enzymatic treatment using amylase and cellulase complex. The duckweed-derived glucose was utilized for polyhydroxybutyrate (PHB) production in engineered Escherichia coli. In addition, the low concentration supplementation of the duckweed extract promoted cell growth compared to the analytical grade glucose.