Maintaining the differentiated phenotype and function of primary hepatocytes and represents a distinct challenge. Our paper describes microcapsules comprised of a bioactive polymer and overcoated with an ultrathin film as a means of maintaining the function of entrapped hepatocytes for at least two weeks. We previously demonstrated that heparin (Hep)-based microcapsules improved the function of entrapped primary hepatocytes by capturing and releasing cell-secreted inductive signals, including hepatocyte growth factor (HGF). Further enhancement of hepatic function could be gained by loading exogenous HGF into microcapsules. In this study, we demonstrate that an ultrathin coating of tannic acid (TA) further enhances endogenous HGF signaling for entrapped hepatocytes and increases by 2-fold the rate of uptake of exogenous HGF by Hep microcapsules. Hepatocytes in overcoated microcapsules exhibited better function and hepatic gene expression than in capsules without a TA coating. Our study showcases the potential application of ultrathin coatings to modulate the bioactivity of microcapsules and may enable the use of encapsulated hepatocytes for modeling drug toxicity or treating liver diseases.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702863 | PMC |
| http://dx.doi.org/10.1021/acsami.4c08329 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
2D/1D Hierarchical Hollow NiO@PPy Composites with Tunable Dielectric Properties for Enhanced Electromagnetic Wave Absorption.
Small
January 2025
College of Chemical Engineering, Fuzhou University, Fuzhou, Fujian, 350116, China.
The development of diverse microstructures has substantially contributed to recent progress in high-performance electromagnetic wave (EMW) absorption materials, providing a versatile platform for the modulation of absorption properties. Exploring multidimensional microstructures and developing tailored and gentle strategies for their precise optimization can substantially address the current challenges posed by relatively unclear underlying mechanisms. Here, a series of 2D/1D heterogeneous NiO@PPy composites featuring hollow hierarchical microstructures are successfully synthesized using a straightforward strategy combining sacrificial templating with chemical oxidative polymerization.
View Article and Find Full Text PDFAnchorable Polymers Enabling Ultra-Thin and Robust Hole-Transporting Layers for High-Efficiency Inverted Perovskite Solar Cells.
Angew Chem Int Ed Engl
January 2025
Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.
Currently, the development of polymeric hole-transporting materials (HTMs) lags behind that of small-molecule HTMs in inverted perovskite solar cells (PSCs). A critical challenge is that conventional polymeric HTMs are incapable of forming ultra-thin and conformal coatings like self-assembly monolayers (SAMs), especially for substrates with rough surface morphology. Herein, we address this challenge by designing anchorable polymeric HTMs (CP1 to CP5).
View Article and Find Full Text PDFMulticolored Bifacial Perovskite Solar Cells through Top Electrode Engineering.
ACS Appl Mater Interfaces
January 2025
Department of Microelectronic Science and Engineering, Ningbo University, Ningbo 315000, China.
Power generation and architectural beauty are equally important for designing efficient and esthetically appealing bifacial perovskite solar cells (PSCs). In this work, efficient and multicolored p-i-n-structured PSCs are achieved by taking advantage of a dielectric/metal/dielectric (DMD)-type (MoO/Ni/Ag/MoO) transparent counter electrode. The MoO/Ni underlayer effectively promotes the formation of a continuous and conductive ultrathin Ag transparent film, especially the 1 nm Ni seed layer adjusts the interface energy level between perovskite/MoO and Ag, resulting in Ohmic contact of the electrode to promote charge extraction and collection.
View Article and Find Full Text PDFUnlocking dual-mode enzyme activities on bacterial surface: Directional recognition and swift capture of Alicyclobacillus acidoterrestris from fruit juices.
Food Res Int
January 2025
State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, Liaoning, China. Electronic address:
The acidophilic and heat-resistant characteristics of Alicyclobacillus acidoterrestris (A. acidoterrestris) pose significant challenges to fruit juice production. Traditional thermal removal methods are often ineffective against this resilient bacterium.
View Article and Find Full Text PDFUltrathin zwitterionic COF membranes from colloidal 2D-COF towards precise molecular sieving.
Water Res
December 2024
College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China. Electronic address:
Membrane technology is an important component of resource recovery. Covalent organic frameworks (COFs) with inherent long-range ordered structure and permanent porosity are ideal materials for fabricating advanced membrane. Zwitterionic COFs have unique features beyond single ionic COFs containing anions or cations.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!