AI Article Synopsis
- - The study highlights the issue of decreasing eradication rates of infections due to antibiotic resistance and suggests that personalized treatment based on a patient's unique metabolic capabilities and antibiotic resistance could improve outcomes.
- - Researchers developed a targeted next-generation sequencing panel, NGS-PHET, to analyze antibiotic resistance variants and host metabolism, leading to tailored treatment recommendations for individual patients.
- - The results showed that personalized therapy recommendations matched actual eradication outcomes for nine out of twelve patients and demonstrated that this method can effectively address antibiotic-resistant strains in clinical and research settings.
Article Abstract
Introduction: The decreasing eradication rate is primarily attributed to antibiotic resistance, and further exacerbated by uniform drug administration disregarding a host's metabolic capability. Consequently, applying personalized treatment based on antibiotic resistance-associated variants and the host's metabolic phenotype can potentially increase the eradication rate.
Method: A custom next-generation sequencing panel for personalized eradication treatment (NGS-PHET) was designed which targeted the regions for amoxicillin, clarithromycin, metronidazole, tetracycline, and levofloxacin-resistance in and human proton-pump inhibitor (PPI) metabolism. The libraries were constructed following customized methods and sequenced simultaneously. The customized framework criteria, grounded in previously reported antibiotic resistance associated variants and the host's PPI metabolism, was applied to the NGS-PHET results and suggested a personalized treatment for each subject, which was validated through each subject's actual eradication outcome.
Results: Both previously reported and novel variants were identified from sequencing results. Concurrently, five homozygous extensive metabolizers and three intermediate metabolizers were identified. Among the total of 12 subjects, clarithromycin triple therapy was suggested for five subjects, bismuth quadruple therapy was suggested for six subjects, and rifabutin triple therapy was suggested for one subject by following the customized framework criteria. The treatment suggestion for nine of the 12 subjects was consistent with the treatment that each subject achieved eradication with.
Discussion: Applying the methodology using the NGS-PHET and customized framework helps to perform eradication treatment quickly and effectively in most patients with antibiotic-resistant strains, and is also useful in research to find novel antibiotic-resistance candidates.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11390507 | PMC |
| http://dx.doi.org/10.3389/fcimb.2024.1379790 | DOI Listing |
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