Antifungal activities of Equol against and .

Virulence

School of Basic Medical Science, Southwest Medical University, Luzhou, People's Republic of China.

Published: December 2024

is an opportunistic fungal pathogen that can cause systemic infections in immunocompromised individuals. Morphological transition and biofilm formation are major virulence factors of . Moreover, biofilm enhances resistance to antifungal agents. Therefore, it is urgent to identify new and effective compounds to target the biofilm of . In the present study, the antifungal activities of equol against were investigated. , the microdilution analysis and spot assay result showed that equol exhibited potent inhibitory activities against . Further investigations confirmed that the antifungal effects of equol involved interference with the transition from yeast to hypha and biofilm formation of . In addition, transcriptome sequencing and reverse transcription-quantitative PCR (qRT-PCR) analysis showed that equol significantly downregulated the expression of several genes in the Ras1-cAMP-PKA pathway related to hyphae and biofilm formation and significantly upregulated the expression of the negative transcriptional repressors and . Moreover, equol effectively reduced the production of cAMP, a key messenger in the Ras1-cAMP-PKA pathway, while supplementation with cAMP partly rescued the equol-induced defects in hyphal development. Furthermore, in a mouse model of systemic candidiasis (SC), equol treatment significantly decreased the fungal burden (liver, kidneys, and lung) in mice and local tissue damage, while enhancing the production of interleukin-10 (IL-10). Together, these findings confirm that equol is a potentially effective agent for treatment of SC.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11409501PMC
http://dx.doi.org/10.1080/21505594.2024.2404256DOI Listing

Publication Analysis

Top Keywords

biofilm formation
12
antifungal activities
8
equol
8
activities equol
8
ras1-camp-pka pathway
8
biofilm
5
antifungal
4
equol opportunistic
4
opportunistic fungal
4
fungal pathogen
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!