AI Article Synopsis
- CRISPR-Cas technology is a powerful tool that helps scientists change the genes of tiny microbes in our bodies, especially in the gut!
- This technology can create better probiotics, find and fight germs that are hard to treat, and improve how well these helpful germs can survive in our stomachs!
- While CRISPR-Cas has many benefits, there are important rules and ethical questions to consider to make sure it's used safely and responsibly!
Article Abstract
CRISPR-Cas technology has revolutionized microbiome research by enabling precise genetic manipulation of microbial communities. This review explores its diverse applications in gut microbiome studies, probiotic development, microbiome diagnostics, pathogen targeting, and microbial community engineering. Engineered bacteriophages and conjugative probiotics exemplify CRISPR-Cas's capability for targeted bacterial manipulation, offering promising strategies against antibiotic-resistant infections and other gut-related disorders. CRISPR-Cas systems also enhance probiotic efficacy by improving stress tolerance and colonization in the gastrointestinal tract. CRISPR-based techniques in diagnostics enable early intervention by enabling fast and sensitive pathogen identification. Furthermore, CRISPR-mediated gene editing allows tailored modification of microbial populations, mitigating risks associated with horizontal gene transfer and enhancing environmental and health outcomes. Despite its transformative potential, ethical and regulatory challenges loom large, demanding robust frameworks to guide its responsible application. This chapter highlights CRISPR-Cas's pivotal role in advancing microbiome research toward personalized medicine and microbial therapeutics while emphasizing the imperative of balanced ethical deliberations and comprehensive regulatory oversight.
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| http://dx.doi.org/10.1016/bs.pmbts.2024.07.008 | DOI Listing |
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