Background: Grounded theory (GT) has become one of the foremost tools in qualitative nursing research. There are different approaches to GT but a feature common to all of them is theoretical sensitivity, which facilitates GT's iterative process. However, differences between the approaches in how to apply theoretical sensitivity and how much influence existing knowledge should play have contributed to tribalism.
Aim: To critically evaluate the role of theoretical sensitivity and reflexivity in GT and the involvement they can have, as well as explore what steps researchers can take to improve their insight.
Discussion: Theoretical sensitivity enables researchers to steer their studies to answer their research questions, gain insight into their study's findings and develop theory grounded in the data. However, reflection is required for researchers to understand their effect on the theories that emerge, prevent them from applying preconceived ideas and allow for the unfettered emergence of theory.
Conclusion: Researchers who do not demonstrate insight into their own philosophical positions and influences risk being accused of bias; this may result in the perceived value of their theoretical outcomes being reduced. Applying a reflexive process may mitigate this, enabling them to understand and refine their methodological processes and produce high-quality GT research.
Implications For Practice: All researchers should consider using reflexivity when conducting research. Understanding influences and positionality in qualitative methodologies allows for transparency and improves the rigour of their outcomes.
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http://dx.doi.org/10.7748/nr.2024.e1936 | DOI Listing |
Talanta
December 2024
Department of Food Science and Engineering, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Food Macromolecules Science and Processing, Shenzhen University, Shenzhen 518060, China. Electronic address:
The simultaneous detection and removal of Ag from drinking water was crucial for preventing human health, while it was also extremely challenging due to bifunctional materials that combine both Ag adsorption and detection functions rarely being explored. In this study, a benzotrithiophene-based covalent organic framework (TAPA-BTT) was synthesized and applied to detect and remove Ag. TAPA-BTT exhibited high crystallinity, a large specific surface area, and good thermal stability.
View Article and Find Full Text PDFSci Total Environ
January 2025
Department of Environmental Science and Engineering, Fudan University, Shanghai, PR China.
The outbreak of cyanobacterial blooms poses an increasingly serious ecological challenge. Our previous study found that calcium peroxide (CaO) has a high inhibitory effect on cyanobacteria, along with a practical application potential in cyanobacteria-dominated lakes. In order to explore the sensitivity of aquatic ecosystems to CaO treatment, we conducted this study to elucidate the ecological impact of CaO on Vallisneria natans (V.
View Article and Find Full Text PDFBMC Plant Biol
January 2025
Agricultural College, Faculty of Agricultural College, Inner Mongolia Agricultural University, Hohhot, 010019, China.
Background: Drought stress is a major environmental constraint affecting crop yields. Plants in agricultural and natural environments have developed various mechanisms to cope with drought stress. Identifying genes associated with drought stress tolerance in potato and elucidating their regulatory mechanisms is crucial for the breeding of new potato germplasms.
View Article and Find Full Text PDFAnal Bioanal Chem
January 2025
College of Chemistry and Chemical Engineering, Linyi University, Linyi, 276000, China.
A molecular beacon is an oligonucleotide hybridization probe that can report the presence of specific nucleic acids in homogeneous solutions. Using an aptamer has allowed an aptamer-based molecular beacon-aptamer beacon to be developed, which has shown advantages of simplicity, rapidity, and sensitivity in imaging and sensing non-nucleic acid substances. However, due to requirement for a deliberate DNA hairpin structure for the preparation of a molecular beacon, not any given aptamer is suitable for designing an aptamer beacon probe.
View Article and Find Full Text PDFNat Struct Mol Biol
January 2025
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Transcription factors (TFs) recognize specific bases within their DNA-binding motifs, with each base contributing nearly independently to total binding energy. However, the energetic contributions of particular dinucleotides can deviate strongly from the additive approximation, indicating that some TFs can specifically recognize DNA dinucleotides. Here we solved high-resolution (<1 Å) structures of MYF5 and BARHL2 bound to DNAs containing sets of dinucleotides that have different affinities to the proteins.
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