Assessing the transepithelial resistance to ion flow in the presence of an electric field enables the evaluation of the integrity of the epithelial cell layer. In this study, we introduce an organic electrochemical transistor (OECT) interfaced with a 3D living tissue, designed to monitor the electrical resistance of cellular barriers in real-time. We have developed a non-invasive, tissue-sensing platform by integrating an inkjet-printed large-area OECT with a 3D-bioprinted multilayered airway tissue. This unique configuration enables the evaluation of epithelial barrier integrity through the dynamic response capabilities of the OECT. Our system effectively tracks the formation and integrity of 3D-printed airway tissues in both liquid-liquid and air-liquid interface culture environments. Furthermore, we successfully quantified the degradation of barrier function due to influenza A (H1N1) viral infection and the dose-dependent efficacy of oseltamivir (Tamiflu®) in mitigating this degradation. The tissue-electronic platform offers a non-invasive and label-free method for real-time monitoring of 3D artificial tissue barriers, without disturbing the cellular biology. It holds the potential for further applications in monitoring the structures and functions of 3D tissues and organs, significantly contributing to the advancement of personalized medicine.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.biomaterials.2024.122806 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Ventilated Three-Dimensional Artificial Lung System for Human Inhalation Exposure Studies.
Environ Sci Technol
December 2024
Department of Environmental Health Sciences, Jonathan and Karin Fielding School of Public Health, University of California, Los Angeles, California 90095, United States.
Traditional in vitro and in vivo models for inhalation toxicology studies often fail to replicate the anatomical and physiological conditions of the human lung. This limitation hinders our understanding of intrapulmonary exposures and their related health effects. To address this gap, we developed a ventilated artificial lung system that replicates human inhalation exposures in four key aspects: (1) facilitating continuous breathing with adjustable respiratory parameters; (2) distributing inhaled aerosols through transitional airflow fields in 3D-printed airway structures, which enables size-dependent particle deposition; (3) duplicating the warm and humid lung environment to promote inhaled aerosol dynamics, such as hygroscopic growth; and (4) supporting the cultivation of human airway epithelium for aerosol exposure and toxicological analyses.
View Article and Find Full Text PDF3D printed drug-eluting stents: Toward personalized therapy for airway stenosis.
J Control Release
January 2025
Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland. Electronic address:
Airway stents are life-saving medical devices used to treat malignant or benign central airway stenoses. Unfortunately, these devices, typically manufactured from silicone alone and/or nitinol, can result in serious complications, such as stent migration, bacterial colonization, and tissue granulation, eventually forcing stent removal. Customized airway stents exhibit reduced migration and they can be tailored to address other complications by releasing drugs, such as the antibiotic levofloxacin and the antifibrotic drug nintedanib.
View Article and Find Full Text PDFComparison of bougie-guided cricothyrotomy and traditional cricothyrotomy techniques in an obese 3D-printed surgical airway manikin: a randomized controlled study.
BMC Anesthesiol
November 2024
Department of Emergency Medicine, Gazi University Hospital, Ankara, Türkiye.
Article Synopsis
- The study investigates the effectiveness of bougie-guided cricothyrotomy versus traditional surgical cricothyrotomy in emergency airway management for obese patients using a 3D-printed manikin.
- It involved 24 emergency residents who practiced both techniques, recording success rates, procedure times, and difficulty scores.
- Results indicated that bougie-guided cricothyrotomy had a higher first-attempt success rate and was considered easier, especially for less experienced residents, though overall success rates and procedure times were similar for both methods.
Bending the rules: A novel approach to laryngeal surgery in a body donor study.
Head Neck
September 2024
Department of Oto-Rhino-Laryngology, University Hospital Heidelberg, Heidelberg, Germany.
Background: Transoral laser microsurgery, the standard surgical approach for early-stage laryngeal cancer, necessitates an unobstructed line of sight to the operating field. However, achieving adequate laryngeal exposure can be challenging, potentially compromising treatment outcomes.
Methods: We developed a 3D-printed curved laryngoscope (sMAC), designed to match the upper airway anatomy.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!