Developing selective and sensitive fluorescent probes for the detection of glutathione (GSH) concentration and intracellular distribution is of great significance for early diagnosis and treatment of diseases such as liver injury and cancer since GSH plays irreplaceable roles in regulating intracellular redox homeostasis. Herein, we present a new fluorescent probe that can be specifically activated by GSH through the conjugate addition and hydrolysis induced covalent-assembly approach for achieving zero-background interference fluorescence off-on sensing. Besides, the probe exhibited prominent selectivity and sensitivity, a low detection limit and cytotoxicity, thus successfully realizing specific real-time monitoring and tracking of GSH levels in living cells. As a consequence, this work might provide a potentially promising candidate for validating the function of GSH in various physiological and pathological processes, which is beneficial for early diagnosis and therapeutics of related diseases.
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