A series of 6 small PAMAM dendrimers (G0-dendrimers) differing in the size, polarity and flexibility has been synthesized. The toxicity has been investigated in three different human cancer cell lines (HeLa, MCF-7, THP-1) and the endothelial skin cell line HMEC-1 in order to evaluate their potential as vehicles for drug delivery.
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http://dx.doi.org/10.1039/d4ra02020k | DOI Listing |
J Phys Chem B
January 2025
Institute for Polymer Research, Waterloo Institute for Nanotechnology, Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
The conformation of a series of zero-generation polyamidoamine dendrimers end-labeled with four 1-pyrene-butyroyl, -hexanoyl, -octanoyl, -decanoyl, and -dodecanoyl derivatives, referred to as the PyCX-PAMAM-G0 samples with = 4, 6, 8, 10, and 12, respectively, was characterized in ,-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), and aqueous solutions of 50 mM sodium dodecyl sulfate (SDS) or 50 mM dodecyltrimethylammonium bromide (DTAB). The conformation of the PyCX-PAMAM-G0 samples was determined from the global model-free analysis (MFA) of the fluorescence decays, which yielded the average rate constant (⟨⟩) for pyrene excimer formation (PEF) between an excited and a ground-state pyrenyl labels, with ⟨⟩ being proportional to the local concentration ([Py]) of the pyrenyl labels within the macromolecular volume; ⟨⟩-vs-[Py] plots yielded straight lines passing through the origin in DMF and DMSO, demonstrating that the internal segments of the dendrimers obeyed Gaussian statistics in these two solvents. In aqueous surfactant solutions, the hydrophobic pyrenyl labels induced the interactions of the PyCX-PAMAM-G0 dendrimers with the SDS and DTAB micelles.
View Article and Find Full Text PDFPharmaceutics
November 2024
The National Dendrimer & Nanotechnology Center, NanoSynthons LLC, Mt. Pleasant, MI 48858, USA.
This perspective begins with an overview of the major impact that the dendron, dendrimer, and dendritic state (DDDS) discovery has made on traditional polymer science. The entire DDDS technology is underpinned by an unprecedented new polymerization strategy referred to as step-growth, amplification-controlled polymerization (SGACP). This new SGACP paradigm allows for routine polymerization of common monomers and organic materials into precise monodispersed, dendritic macromolecules (i.
View Article and Find Full Text PDFPharmaceutics
November 2024
Centro de Investigaciones Químicas, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca CP 62209, Mexico.
Doxorubicin (Dox) is an anticancer drug used in the treatment of a wide range of solid tumors; however, Dox causes systemic toxicity and irreversible cardiotoxicity. The design of a new nanosystem that allows for the control of Dox loading and delivery results is a powerful tool to control Dox release only in cancer cells. For this reason, supramolecular self-assembly was performed between a poly(amidoamine) (PAMAM) dendrimer decorated with four β-cyclodextrin (βCD) units (PAMAM-βCD) and an adamantane-hydrazone-doxorubicin (Ad-h-Dox) prodrug.
View Article and Find Full Text PDFPolymers (Basel)
December 2024
School of Materials Science and Engineering, Changzhou University, Changzhou 213164, China.
Amide-amine (PAMAM) dendrimers are biodegradable, non-immunogenic, genotoxic, and biocompatibible, which make them excellent materials for biological applications. In order to reduce the cytotoxicity of the designed branched molecules, a four-armed branched nucleus (B4) of PAMAM dendrimers as hyperbranched molecules was fused with polyhexamethylene biguanide (PHMB) (A2); hyperbranched polymeric biguanides (PAPBs) with a four-arm central core PAMAM structure were synthesized. The bactericidal and cell toxicity tests showed that PAPB had excellent bactericidal activity against both Gram-positive bacteria and Gram-negative, and the chemical binding of PHMB and PAMAM had synergistic effects.
View Article and Find Full Text PDFACS Omega
December 2024
Department of Biochemistry, Federal University of São Paulo, São Paulo, SP 04044-020, Brazil.
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