Similar Publications

Probing Macromolecular Conformation in Restricted Geometry by PEF: Application to Hydrophobically Modified PAMAM Dendrimers Isolated Inside Surfactant Micelles.

J Phys Chem B

January 2025

Institute for Polymer Research, Waterloo Institute for Nanotechnology, Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.

The conformation of a series of zero-generation polyamidoamine dendrimers end-labeled with four 1-pyrene-butyroyl, -hexanoyl, -octanoyl, -decanoyl, and -dodecanoyl derivatives, referred to as the PyCX-PAMAM-G0 samples with = 4, 6, 8, 10, and 12, respectively, was characterized in ,-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), and aqueous solutions of 50 mM sodium dodecyl sulfate (SDS) or 50 mM dodecyltrimethylammonium bromide (DTAB). The conformation of the PyCX-PAMAM-G0 samples was determined from the global model-free analysis (MFA) of the fluorescence decays, which yielded the average rate constant (⟨⟩) for pyrene excimer formation (PEF) between an excited and a ground-state pyrenyl labels, with ⟨⟩ being proportional to the local concentration ([Py]) of the pyrenyl labels within the macromolecular volume; ⟨⟩-vs-[Py] plots yielded straight lines passing through the origin in DMF and DMSO, demonstrating that the internal segments of the dendrimers obeyed Gaussian statistics in these two solvents. In aqueous surfactant solutions, the hydrophobic pyrenyl labels induced the interactions of the PyCX-PAMAM-G0 dendrimers with the SDS and DTAB micelles.

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This perspective begins with an overview of the major impact that the dendron, dendrimer, and dendritic state (DDDS) discovery has made on traditional polymer science. The entire DDDS technology is underpinned by an unprecedented new polymerization strategy referred to as step-growth, amplification-controlled polymerization (SGACP). This new SGACP paradigm allows for routine polymerization of common monomers and organic materials into precise monodispersed, dendritic macromolecules (i.

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Using Poly(amidoamine) PAMAM-βCD Dendrimer for Controlled and Prolonged Delivery of Doxorubicin as Alternative System for Cancer Treatment.

Pharmaceutics

November 2024

Centro de Investigaciones Químicas, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca CP 62209, Mexico.

Doxorubicin (Dox) is an anticancer drug used in the treatment of a wide range of solid tumors; however, Dox causes systemic toxicity and irreversible cardiotoxicity. The design of a new nanosystem that allows for the control of Dox loading and delivery results is a powerful tool to control Dox release only in cancer cells. For this reason, supramolecular self-assembly was performed between a poly(amidoamine) (PAMAM) dendrimer decorated with four β-cyclodextrin (βCD) units (PAMAM-βCD) and an adamantane-hydrazone-doxorubicin (Ad-h-Dox) prodrug.

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Amide-amine (PAMAM) dendrimers are biodegradable, non-immunogenic, genotoxic, and biocompatibible, which make them excellent materials for biological applications. In order to reduce the cytotoxicity of the designed branched molecules, a four-armed branched nucleus (B4) of PAMAM dendrimers as hyperbranched molecules was fused with polyhexamethylene biguanide (PHMB) (A2); hyperbranched polymeric biguanides (PAPBs) with a four-arm central core PAMAM structure were synthesized. The bactericidal and cell toxicity tests showed that PAPB had excellent bactericidal activity against both Gram-positive bacteria and Gram-negative, and the chemical binding of PHMB and PAMAM had synergistic effects.

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Article Synopsis
  • PAMAM dendrimers, known for their structural versatility and customizable surfaces, are being investigated for biomedical uses, but their interactions with blood can disrupt normal clotting and pose health risks.
  • The study focused on how low-generation PAMAM dendrimers affect fibrin clot formation dynamics, including clot structure and resistance to breakdown, using various methods and blood samples.
  • Notably, certain dendrimers like G2-NH and G4-NH hindered clot formation and altered clot properties significantly, while G3.5-COOH showed minimal impact, suggesting it could be a safer option for medical applications.
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