AI Article Synopsis
- PMT (Pasteurella multocida toxin) is a major virulence factor in type D infections, leading to significant economic losses in pig farming and severe organ damage in both pigs and mice.
- The study found that PMT is highly cytotoxic, particularly affecting the kidneys and lungs, with even low doses causing weight loss and higher doses resulting in acute death in both mice and pigs.
- Pathological analysis revealed severe organ damage, including hemorrhage in the lungs and liver, and desolation of the kidneys, highlighting PMT's extreme toxicity and its impact on vital organs.
Article Abstract
toxin (PMT) is one of the most important virulence factors of type D. infection has caused enormous economic losses in the pig farming industry. Although it is well known that this bacterial infection causes progressive atrophic rhinitis, its effects on other organ tissues in pigs are unclear. In this study, PMT was expressed and purified, and the cytotoxic effects of PMT on four types of swine cells, LLC-PK1, PAM, IPEC, and ST, were investigated. LLC-PK1 exhibited the highest sensitivity to the cytotoxic effects of PMT. Our studies revealed that a PMT concentration of 0.1 μg/kg can lead to weight loss, whereas a PMT concentration of 0.5 μg/kg can lead to death in mice. PMT causes damage to the intestines, kidneys, lungs, livers, and spleens of mice. Furthermore, PMT caused acute death in pigs at treatment concentrations greater than 5 μg/kg; at PMT concentration of 2.5 μg/kg, weight loss occurred until death. PMT mainly caused damage to the hearts, lungs, livers, spleens and kidneys of pigs. The organ coefficient showed that damage to the heart and kidneys was the most severe and caused the renal pelvis and renal pyramid to dissolve and become cavitated. Pathology revealed hemorrhage in the lungs, liver, and spleen, and the kidneys were swollen and vacuolated, which was consistent with the damaged target organs in the mice. In conclusion, these findings demonstrate that PMT is extremely toxic and , causing damage to various organs of the body, especially the kidneys and lungs. This study provides a theoretical basis for the in-depth exploration of the cytotoxic effects of PMT on target organs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11385013 | PMC |
| http://dx.doi.org/10.3389/fmicb.2024.1459124 | DOI Listing |
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