The first molecular record of , , , s.s. and in Bosnia and Herzegovina.

sensu lato complex comprises 20 species, from which , , sensu stricto, , and are directly associated with Lyme borreliosis, while , , and were detected in individual cases. Their main vector in Europe is the hard tick species . To date, two species, and , have been molecularly detected in Bosnia and Herzegovina. To test for the presence of other species, we performed nested PCR targeting intergenic region on DNA isolates from 49 ticks collected from vegetation by flagging method and 43 removed from humans in The Center for Emergency Medical Assistance of the Sarajevo Canton and The Healthcare Centers of the Sarajevo Canton. species were detected by one-directional Sanger sequencing of the amplified region using the same forward primer as in PCR. Out of six species detected in the present study, this is the first record of , , . , and in Bosnia and Herzegovina.