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Genome-wide identification of oxidosqualene cyclase genes regulating natural rubber in Taraxacum kok-saghyz. | LitMetric

Genome-wide identification of oxidosqualene cyclase genes regulating natural rubber in Taraxacum kok-saghyz.

Planta

Key Laboratory of Tropical Islands Ecology, Ministry of Education, College of Life Sciences, Hainan Normal University, Haikou, 571158, People's Republic of China.

Published: September 2024

AI Article Synopsis

  • Nine TkOSC genes were discovered in Taraxacum kok-saghyz through genome-wide screening, with TkOSC4-6 likely being key players in natural rubber biosynthesis.
  • The Oxidosqualene cyclase (OSC) family is vital for regulating this process, and their proteins showed high conservation and potential interactions with other important enzymes.
  • Further analysis indicates that TkOSC4-6 have strong expression levels in specific tissues and may play significant roles in natural rubber production in TKS roots.

Article Abstract

Nine TkOSC genes have been identified by genome-wide screening. Among them, TkOSC4-6 might be more crucial for natural rubber biosynthesis in Taraxacum kok-saghyz roots. Taraxacum kok-saghyz Rodin (TKS) roots contain large amounts of natural rubber, inulin, and valuable metabolites. Oxidosqualene cyclase (OSC) is a key member for regulating natural rubber biosynthesis (NRB) via the triterpenoid biosynthesis pathway. To explore the functions of OSC on natural rubber producing in TKS, its gene family members were identified in TKS genome via genome-wide screening. Nine TkOSCs were identified, which were mainly distributed in the cytoplasm. Their family genes experienced a neutral selection during the evolution process. Overall sequence homology analysis OSC proteins revealed 80.23% similarity, indicating a highly degree of conservation. Pairwise comparisons showed a multiple sequence similarity ranging from 57% to 100%. Protein interaction prediction revealed that TkOSCs may interact with baruol synthase, sterol 1,4-demethylase, lupeol synthase and squalene epoxidase. Phylogenetic analysis showed that OSC family proteins belong to two branches. TkOSC promoter regions contain cis-acting elements related to plant growth, stress response, hormones response and light response. Protein accumulation analysis demonstrated that TkOSC4, TkOSC5 and TkOSC6 proteins had strong expression levels in the root, latex and plumular axis. Comparison of gene expression patterns showed TkOSC1, TkOSC4, TkOSC5, TkOSC6, TkOSC7, TkOSC8 and TkOSC9 might be important in regulating NRB. Combination of gene and protein results revealed TkOSC4-6 might be more crucial, and the data might contribute to a more profound understanding of the roles of OSCs for NRB in TKS roots.

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Source
http://dx.doi.org/10.1007/s00425-024-04522-yDOI Listing

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