H, C and N backbone resonance assignment of the calcium-activated EndoU endoribonuclease.

Biomol NMR Assign

Univ. Bordeaux, CNRS, INSERM, ARNA, UMR 5320, U1212, Bordeaux, F-33000, France.

Published: December 2024

The catalytic domain of the calcium-dependent endoribonuclease EndoU from Homo sapiens was expressed in E. coli with C and N labeling. A nearly complete assignment of backbone H, N, and C resonances was obtained, as well as a secondary structure prediction based on the assigned chemical shifts. The predicted secondary structures were almost identical to the published crystal structure of calcium-activated EndoU. This is the first NMR study of an eukaryotic member of the EndoU-like superfamily of ribonucleases.

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http://dx.doi.org/10.1007/s12104-024-10198-yDOI Listing

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