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A zinc metal complex as an NIR emissive probe for real-time dynamics and embryogenic evolution of lysosomes using super-resolution microscopy. | LitMetric

AI Article Synopsis

  • - Zinc (Zn) based fluorescent metal complexes are gaining attention for their non-toxicity and high brightness, but have not been widely used in super-resolution microscopy (SRM) for studying live cells, particularly lysosomes.
  • - A new highly photostable Zn-complex was developed as a fluorescent probe to map the dynamics and distribution of lysosomes in both cancerous and non-cancerous cells, revealing notable differences in motility linked to cytoplasmic viscosity.
  • - The study successfully captured super-resolved images of lysosomes and lysosome-related organelles (LROs) throughout embryonic development, from a single cell to a fully matured adult organism, highlighting the complex changes in these structures over time. *

Article Abstract

Zinc (Zn) based fluorescent metal complexes have gained increasing attention due to their non-toxicity and high brightness with marked fluorescence quantum yield (QY). However, they have rarely been employed in super-resolution microscopy (SRM) to study live cells and dynamics of lysosomes. Here, we present an NIR emissive highly photostable Zn-complex as a multifaceted fluorescent probe for the long-term dynamical distribution of lysosomes in various cancerous and non-cancerous cells in live condition and embryogenic evolution in (). Apart from the normal fission, fusion, and kiss & run, the motility and the exact location of lysosomes at each point were mapped precisely. A notable difference in the lysosomal motility in the peripheral region between cancerous and non-cancerous cells was distinctly observed. This is attributed to the difference in viscosity of the cytoplasmic environment. On the other hand, along with the super-resolved structure of the smallest size lysosome (∼77 nm) in live , the complete embryogenic evolution of lysosomes and lysosome-related organelles (LROs) was captured. We were able to capture the images of lysosomes and LROs at different stages of , starting from a single cell and extending to a fully matured adult animal.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11376271PMC
http://dx.doi.org/10.1039/d4sc04638bDOI Listing

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