AI Article Synopsis
- The study investigates the expression of SOX17 and other immunohistochemical markers in mesonephric-like adenocarcinoma (MLA) to help distinguish it from other endometrial carcinomas.
- All 17 collected MLA samples exhibited strong positive PAX8 staining, negative ER, and variable TTF1/GATA3 staining, while SOX17 showed weak or no expression, contrasting with other carcinoma types.
- The findings suggest that using a combination of SOX17 and PAX8 in diagnostic tests could effectively identify MLAs, particularly when strong PAX8 and negative SOX17 results are observed.
Article Abstract
Aims: Mesonephric-like adenocarcinoma (MLA) of the endometrium or ovary is a rare but distinct endometrial carcinoma which has a combination of characteristic morphological, immunohistochemical (IHC) and molecular features. SOX17 has been recently identified as a highly sensitive and specific marker for endometrial and ovarian carcinomas. In this study, we aimed to investigate SOX17 expression in MLA together with other IHCs to differentiate MLAs from other endometrial carcinomas.
Methods: Seventeen previously diagnosed endometrial/ovarian MLAs were collected, and multiple IHCs were performed. Additionally, we performed SOX17, PAX8 and ER on tissue microarrays (TMAs) composed of 652 endometrial carcinomas from 2012 to 2015 when MLA diagnostic criteria were not established.
Results: All 17 MLAs showed diffuse strong positive PAX8, negative ER and variable TTF1/GATA3 staining. Notably, all MLAs showed negative (n = 10) or focal weak/moderate (n = 7) staining for SOX17, which is more diffuse and stronger than PAX8 in other endometrial carcinoma subtypes. This finding prompted us to screen TMAs with 652 endometrial carcinomas diagnosed before MLA by an approach of combined SOX17 and PAX8 IHCs, and 14 cases with positive PAX8 but negative/focal weak SOX17 were identified. We further studied the 14 cases by examining morphology and performing additional IHCs (TTF1, GATA3, ER and CD10) and would classify seven (50%) of them as MLAs based on morphological features and positive CD10, TTF1 and/or GATA3 staining.
Conclusion: Our results suggest that a combination of SOX17 and PAX8 IHCs would aid in diagnosing MLA if the results show strong positive PAX8, but negative SOX17.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11649513 | PMC |
| http://dx.doi.org/10.1111/his.15312 | DOI Listing |
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