AI Article Synopsis

  • - This study aimed to validate a new rRT-PCR assay that can rapidly and simultaneously detect African swine fever virus and classical swine fever virus without needing to purify viral nucleic acids.
  • - Testing was conducted on 101 pig samples from Vietnam collected between 2018 and 2021, with the assay showing a diagnostic sensitivity of 98.8% and 100% specificity.
  • - Results indicated that the assay can accurately use crude samples, making it a promising tool for fast and effective disease detection and management in swine populations.

Article Abstract

Objective: This study validates a direct multiplex real-time reverse transcription polymerase chain reaction (rRT-PCR) assay which was previously established for enabling rapid and simultaneous detection of African swine fever (ASF) virus (ASFV) and classical swine fever virus. The assay eliminates the need for viral nucleic acid purification using a buffer system for crude extraction and an impurity-tolerant enzyme. However, the assay had not yet been validated using field samples of ASFV-infected pigs. Therefore, to address this gap, we tested 101 samples collected from pigs in Vietnam during 2018 and 2021 for validation.

Results: The rRT-PCR assay demonstrated a diagnostic sensitivity of 98.8% and a specificity of 100%. Remarkably, crude samples yielded results comparable to those of purified samples, indicating the feasibility of using crude samples without compromising accuracy in ASFV detection. Our findings emphasize the effectiveness of the rRT-PCR assay for the prompt and accurate diagnosis of both swine fever viruses, which is essential for effective disease prevention and control in swine populations.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11370023PMC
http://dx.doi.org/10.1186/s13104-024-06898-2DOI Listing

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