The green peach aphid, , is a worldwide agricultural pest. Chlorpyrifos has been widely used to control for decades, thus leading to a high resistance to chlorpyrifos. Recent studies have found that insect odorant binding proteins (OBPs) play essential roles in insecticide resistance. However, the potential resistance mechanism underlying the cross-link between aphid OBPs and chlorpyrifos remains unclear. In this study, two OBPs (MperOBP3 and MperOBP7) were found overexpressed in chlorpyrifos-resistant strains (CRR) compared to chlorpyrifos-sensitive strains (CSS); furthermore, chlorpyrifos can significantly induce the expression of both OBPs. An binding assay indicated that both OBPs strongly bind with chlorpyrifos; an RNAi and toxicity bioassay confirmed silencing either of the two OBPs can increase the susceptibility of aphids to chlorpyrifos, suggesting that overexpression of MperOBP3 and MperOBP7 contributes to the development of resistance of to chlorpyrifos. Our findings provide novel insights into insect OBPs-mediated resistance mechanisms.
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http://dx.doi.org/10.1021/acs.jafc.4c05026 | DOI Listing |
Int J Biol Macromol
December 2024
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address:
Odorant binding proteins (OBPs) play key roles in the insect olfactory system by assisting the neuronal response to hydrophobic odor molecules, understanding their interaction with ligands will facilitate the virtual screening of behaviorally active compounds in insects. Here, we successfully cloned and confirmed CmedOBP13, an antennae-biased OBP from the rice leaffolder Cnaphalocrocis medinalis, as a secreted protein. Recombinant CmedOBP13 was obtained using the Escherichia coli system, and its binding affinities to 35 volatile compounds emitted by rice plants and three sex pheromone components from female moths were assessed by a competitive binding assay.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China. Electronic address:
Three Tomicus bark beetles (T. yunnanensis, T. brevipilosus and T.
View Article and Find Full Text PDFJ Hazard Mater
December 2024
State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing 100093, China. Electronic address:
The risk of neonicotinoid insecticides to honeybees is a global issue. Cycloxaprid (CYC) is a novel neonicotinoid insecticide with outstanding activities, good safety profiles, and no cross-resistance with other neonicotinoids. Information on the environmental risks of CYC is limited, especially its effects on honeybees.
View Article and Find Full Text PDFFront Physiol
December 2024
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
The highly developed sensitive olfactory system is essential for Scott (Hemiptera: Pentatomidae) adults, an widely distributed natural predatory enemy, to locate host plants. During this process, odorant-binding proteins (OBPs) are thought to have significant involvement in the olfactory recognition. However, the roles of OBPs in the olfactory perception of are not frequently reported.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
Winged aphids develop more sensitive olfaction than the wingless phenotype to identify potential habitat from afar. Two types of olfactory sensilla, primary rhinarium (PRh) and secondary rhinarium (SRh) are responsible for aphid olfactory perception, of which, SRh is involved in the perception of both E-β-farnesene (EBF) and plant volatiles. Odorant binding proteins (OBPs) play a vital role in the response of insect olfactory nerves located in the rhinarium to external odor stimuli.
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