Sensor Engineering Department, Faculty of Science and Engineering, Maastricht University, P.O. Box 616, Maastricht 6200 MD, The Netherlands.
Published: August 2024
AI Article Synopsis
Article Abstract
is a multidrug-resistant Gram-negative bacterium that poses a significant threat to public health, necessitating rapid and on-site detection methods for rapid recognition. The goal of the project is therefore to indirectly detect the presence of in environmental water samples targeting one of its quorum-sensing molecules, namely, ()--butyryl homoserine lactone (BHL). To this aim, molecularly imprinted polymers (MIPs) were synthesized via bulk free-radical polymerization using BHL as a template molecule. The obtained MIP particles were immobilized onto screen-printed electrodes (MIP-SPEs), and the BHL rebinding was analyzed via electrochemical impedance spectroscopy (EIS). To study the specificity of the synthesized MIPs, isotherm curves were built after on-point rebinding analysis performed via LC-MS measurements for both MIPs and NIPs (nonimprinted polymers, used as a negative control), obtaining an imprinting factor (IF) of 2.8 (at = 0.4 mM). The MIP-SPEs were integrated into an electrochemical biosensor with a linear range of 1 × 10-1 × 10 nM and a limit of detection (LoD) of 31.78 ± 4.08 nM. Selectivity measurements were also performed after choosing specific interferent molecules, such as structural analogs and potential interferents, followed by on-point analysis performed in spiked tap water to prove the sensor's potential to detect the presence of the quorum-sensing molecule in environmentally related real-life samples.
Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling & International Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
* The presence of persistent free radicals (PFRs) in RS biochar contributes to the degradation of a signaling molecule (C4-HSL), which disrupts the communication (quorum sensing) among HBPs.
* This research highlights the potential of biochar as a soil remediation technology to lower the risks posed by HBPs, emphasizing the importance of biochar's source in its effectiveness.
Anaerobic digestion (AD) is a promising technology for achieving both organic wastes treatment and energy recovery. However, challenges such as ammonia inhibition still remain. Quorum sensing (QS) system is relevant with the regulation of microbial community behaviors by releasing and sensing signal molecules, which could improve methane production during AD process.
is a multidrug-resistant Gram-negative bacterium that poses a significant threat to public health, necessitating rapid and on-site detection methods for rapid recognition. The goal of the project is therefore to indirectly detect the presence of in environmental water samples targeting one of its quorum-sensing molecules, namely, ()--butyryl homoserine lactone (BHL). To this aim, molecularly imprinted polymers (MIPs) were synthesized via bulk free-radical polymerization using BHL as a template molecule.
Many Gram-negative bacteria use N-acyl-L-homoserine lactone (AHL) signals to coordinate phenotypes such as biofilm formation and virulence factor production. Quorum-quenching enzymes, such as AHL acylases, chemically degrade these molecules which prevents signal reception by bacteria and inhibits undesirable biofilm-related traits. These capabilities make acylases appealing candidates for controlling microbes, yet candidates with high activity levels and substrate specificity and that are capable of being formulated into materials are needed.
Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
The last step of the initiation phase of fatty acid biosynthesis in most bacteria is catalyzed by the 3-ketoacyl-acyl carrier protein (ACP) synthase III (FabH). pv. strain B728a encodes two FabH homologs, Psyr_3467 and Psyr_3830, which we designated PssFabH1 and PssFabH2, respectively.
