Development of a highly effective recombinant protein from human collagen type III Alpha 1 (COL3A1) to enhance human skin cell functionality.

BMB Rep

Department of Biomedical Sciences, College of Medicine, Inha University, Incheon 22212, 2Program in Biomedical Science & Engineering, Inha University, Incheon 22212; Research Center for Controlling Intercellular Communication (RCIC), College of Medicine, Inha University, Incheon 22212, Korea.

Published: September 2024

AI Article Synopsis

  • Collagen type III is an important protein found in various tissues like skin and blood vessels, essential for their structure and healing process, but its specific effects on skin have not been deeply researched.
  • The study successfully created a recombinant version of collagen type III that promotes cell growth and collagen production in skin cells, aiding wound healing.
  • The engineered protein, known as HUCOLLATIN3, effectively penetrates skin layers and shows potential as a biomaterial to combat skin aging.

Article Abstract

Collagen type III, a member of the fibrillar collagen group, is a major component of the extracellular matrix in various internal organs, the vascular systems, and skin. It is essential to maintain the structural integrity and functionality of these tissues, and plays a significant role in wound healing, often found alongside collagen type I. Despite being the second most abundant collagen in human tissues after type I, its biological functions on various skin properties have not been thoroughly studied. In this study, we have isolated and developed an effective recombinant protein derived from human collagen type III alpha 1 chain (hCOL3A1). Our findings demonstrate that the recombinant proteins hCOL3A1-THR-M1 and M4 stimulate cell proliferation and collagen biosynthesis in human dermal fibroblasts (HDFs), and enhance wound healing. Notably, hCOL3A1-THR- M1 (referred to as HUCOLLATIN3) specifically penetrates both the epidermal and dermal layers in a full-thickness skin model. These results collectively indicate that hCOL3A1-THR-M1 holds promise as a potential biomaterial to prevent skin aging. [BMB Reports 2024; 57(9): 424-429].

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11444990PMC
http://dx.doi.org/10.5483/BMBRep.2024-0107DOI Listing

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