AI Article Synopsis
- - The study investigates microalgae-based glycolate production, highlighting the limitations posed by traditional low-density photoautotrophic cultivation and existing strains.
- - Researchers utilized a targeted knockout method to inactivate a key enzyme (CrHPR1) in Chlamydomonas reinhardtii, resulting in a significant increase in glycolate production, achieving up to 2.1 g/L under optimized conditions.
- - The findings suggest that cell wall-deficient mutants may enhance glycolate excretion, paving the way for advancements in microalgae-based biotechnology and greener industrial processes.
Article Abstract
Microalgae-based glycolate production through the photorespiratory pathway is considered an environmentally friendly approach. However, the potential for glycolate production is limited by photoautotrophic cultivation with low cell density and existing strains. In this study, a targeted knockout approach was used to disrupt the key photorespiration enzyme, Chlamydomonas reinhardtii hydroxypyruvate reductase 1 (CrHPR1), leading to a significant increase in glycolate production of 280.1 mg/L/OD. The highest potency yield reached 2.1 g/L under optimized mixotrophic conditions, demonstrating the possibility of synchronizing cell growth with glycolate biosynthesis in microalgae. Furthermore, the hypothesis that the cell wall-deficient mutant facilitates glycolate excretion was proposed and validated by comparing the glycolate accumulation trends of various Chlamydomonas reinhardtii strains. This study will facilitate the development of microalgae-based biotechnology and shed lights on the continuous advancement of green biomanufacturing for industrial application.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.biortech.2024.131372 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An updated overview on long-acting therapeutics for the prevention and treatment of human immunodeficiency virus (HIV) from a perspective of pharmaceutics.
Int J Pharm
December 2024
Division of Pharmaceutical Sciences, James L. Winkle College of Pharmacy, University of Cincinnati, Cincinnati, OH 45229, USA. Electronic address:
Conventional drug formulations release active pharmaceutical ingredients (APIs) immediately after administration, while long-acting (LA) drug products are designed for prolonged therapeutic effects, thereby reducing administration frequency and improving patient compliance. The development of LA therapeutics for chronic disease treatment has significantly helped patients adhere to their regimens, reducing the need for daily doses and easing the burden on healthcare systems. Advances in treatment have transformed Human Immunodeficiency Virus (HIV) into a manageable chronic disease, and efforts are underway to eliminate HIV in the future.
View Article and Find Full Text PDFFunctionalized Microsphere Platform Combining Nutrient Restriction and Combination Therapy to Combat Bacterial Infections.
ACS Appl Mater Interfaces
January 2025
Animal-Derived Food Safety Innovation Team, College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China.
The escalating prevalence of multidrug-resistant (MDR) bacterial infections has emerged as a critical global health crisis, undermining the efficacy of conventional antibiotic therapies. This pressing challenge necessitates the development of innovative strategies to combat MDR pathogens. Advances in multifunctional drug delivery systems offer promising solutions to reduce or eradicate MDR bacteria.
View Article and Find Full Text PDFEvaluation of Performance and Stability of a Gel-Type Polymer Sorbent for Recovery of Phosphate from Waste Streams.
ACS Appl Polym Mater
December 2024
School of Chemistry and Chemical Engineering, Queen's University, David Keir Building, Stranmillis Road, BT9 5AG Belfast, Northern Ireland, U.K.
Phosphorus (P) fertilizer is an essential component of our food system with the majority of all mined P rock processed to make mineral fertilizers. Globally however P rock stocks are declining-both in quality and quantity-with poor P management creating a linear economic system where P is mined, globally redistributed into products and eventually discharged into the environment leading to eutrophication. To enable establishment of a circular P economy, whereby P can be recovered from waste for its industrial reuse, requires the development of effective P recovery technologies.
View Article and Find Full Text PDFDual silencing of integrin αvβ3 receptor and insulin-like growth factor 1 receptor using mPEG-PCL/DDAB hybrid nanoparticle loaded siRNA in breast cancer therapy: An in vitro study on MCF-7 cells.
Int J Biol Macromol
December 2024
Research Institute for Prevention of Non-Communicable Diseases, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Electronic address:
Integrin αvβ3, a primary cell-adhesion receptor, plays a crucial role in various biological processes, including angiogenesis, pathological neovascularization, and tumor metastasis. Its expression increases during tumor angiogenesis. The insulin-like growth factor 1 receptor (IGF1R) is a transmembrane protein that stimulates vital signaling pathways, promoting cancer cell growth, survival, and metabolism.
View Article and Find Full Text PDFPEG-Polymeric Nanocarriers Alleviate the Immunosuppressive Effects of Free 4-Thiazolidinone-Based Chemotherapeutics on T Lymphocyte Function and Cytokine Production.
Int J Nanomedicine
January 2025
International Research and Innovation in Medicine Program, Cedars - Sinai Medical Center, Los Angeles, CA, USA.
Purpose: Our study aimed to assess the effects of anticancer 4-thiazolidinone-based free water-insoluble therapeutics Les-3288 and Les-3833 and their waterborne complexes with branched PEG-containing polymeric carriers (A24-PEG550 and A24-PEG750) on immune response.
Methods: Human peripheral blood was used to study in vitro lymphocyte proliferative function, leukocyte phagocytic activity and respiratory burst, and cytokine production.
Results: The binding of the polymer to the anticancer drug Les-3288, which is intended to mitigate the immunosuppressive effects of the free drug on the proliferative activity of T lymphocytes and T-dependent B cells, demonstrated comparable efficacy for both A24-PEG750 and A24-PEG550 nanocarriers.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!