AI Article Synopsis
- Alpha-ketoglutarate-dependent dioxygenases (αKGDs) are emerging as effective biocatalysts for oxidizing and modifying carbon-hydrogen (C-H) bonds.
- The study focused on a newly identified αKGD named AneA, which is involved in aculene biosynthesis and shows flexibility in processing various substrates with different ring structures.
- AneA demonstrated the ability to perform both desaturation and hydroxylation reactions, requiring specific amino ester groups on substrates, leading to the creation of a new strategy for synthesizing complex sesquiterpenoids.
Article Abstract
Alpha-ketoglutarate-dependent dioxygenases (αKGDs) have recently emerged as useful biocatalysts for C-H oxidation and functionalization. In this work, we characterized a new αKGD from aculene biosynthesis, AneA, which displays broad promiscuity toward a number of substrates with different ring systems. Unexpectedly, AneA was found to be capable of both desaturation and hydroxylation and require an amino ester motif on its substrate for productive catalysis. Insights gathered from the functional characterization and substrate-activity profiling of AneA enabled the development of a chemoenzymatic strategy toward several complex sesquiterpenoids.
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| http://dx.doi.org/10.1002/anie.202409143 | DOI Listing |
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