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In Vitro Antiprotozoal Activity of Extract, Partitions, and Fractions against . | LitMetric
Chagas disease, caused by the protozoan , represents an important and worldwide public health issue, particularly in Latin America. Limitations of conventional treatment with benznidazole and nifurtimox underscore the urgent need for new therapeutic strategies for this disease. , a tree used in traditional medicine for various ailments, has demonstrated promising antiparasitic activity. The in vitro anti- activity of crude methanol extract, partitions, and fractions, as well as their cytotoxicity in Vero cells and , and hemolytic activity in human erythrocytes were assessed. Most of the extracts possessed anti- effects, with Sm-CF3 being the fraction with the highest activity (IC = 19 µg/mL; SI = 6.8). Gas chromatography-mass spectrometry analysis identified 20 compounds, with fatty acyls comprising the predominant chemical class (55%). We also identified the antiparasitic compounds cis-5,8,11,14,17-eicosapentaenoic acid and trans-Z-α-bisabolene epoxide, suggesting their potential contribution to the observed anti- activity. In conclusion, our findings support the therapeutic potential of as a source of novel antiparasitic agents against .
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