AI Article Synopsis

  • California hare coltivirus (CHCV) was first isolated in 1976, but its distribution and host range were unclear until recent studies in China expanded its known presence.
  • Using next-generation sequencing, researchers found CHCV RNA in 1.56% of tick samples collected from northeastern China, with the highest positivity rates in Inner Mongolia.
  • Genetic analysis showed that current CHCV strains have high identity with previously reported strains and are closely related to human pathogenic coltiviruses, therefore expanding our understanding of CHCV’s diversity and geographic scope.

Article Abstract

California hare coltivirus (CHCV) was isolated in California in 1976 from a hare. Despite its long history, it remained unclear whether CHCV was exclusively distributed in California with limited host ranges. By next-generation sequencing (NGS), we obtained a complete sequence of CHCV from collected in 2019 in northeast China. An expanded epidemiological investigation was subsequently performed on ticks belonging to four species (, , , ) collected in northeastern China by applying CHCV-specific RT-PCR and sequencing. CHCV RNA-positive results were found in 1.56% of the tick samples. Positive ticks were obtained in three of four sampled locations, with the highest rate observed in Inner Mongolia (2.69%), followed by Heilongjiang (1.94%) and Jilin provinces (0.55%). All positive results were derived from ticks (2.33%), while no positive detection was found in the other tick species, even at the same location. Sequence analysis revealed that the current CHCV showed a high genetic identity (>80% amino acid identity) with the previously reported CHCV in all segments except segment seven (64.59% amino acid identity). Phylogenetic analysis based on RNA-dependent RNA polymerase (RdRp) amino acid sequences demonstrated that both the current and previously reported CHCV strains were grouped phylogenetically into the genus . Both CHCV strains formed a distinct clade, clustering with three human pathogenic coltiviruses (Colorado tick fever virus, Salmon River virus, and Eyach virus), and were distant from the other coltiviruses. We report the identification and characterization of CHCV for the first time in ticks, expanding the currently known geographic scope, host, and genetic heterogeneity in CHCV.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11357685PMC
http://dx.doi.org/10.3390/pathogens13080614DOI Listing

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