Photoacoustic imaging (PAI) is an emerging hybrid imaging modality that combines high-contrast optical imaging with high-spatial-resolution ultrasound imaging. PAI can provide a high spatial resolution and significant imaging depth by utilizing the distinctive spectroscopic characteristics of tissue, which gives it a wide variety of applications in biomedicine and preclinical research. In addition, it is non-ionizing and non-invasive, and photoacoustic (PA) signals are generated by a short-pulse laser under thermal expansion. In this study, we describe the basic principles of PAI, recent advances in research in human and animal tissues, and future perspectives.
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http://dx.doi.org/10.3390/mi15081007 | DOI Listing |
Anal Chem
January 2025
State Key Laboratory of Applied Organic Chemistry and Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Gansu, Lanzhou 730000, China.
Mercury ions (Hg) have been found to disrupt the body's antioxidant defense mechanisms, leading to oxidative stress and physiological dysfunction. Early diagnosis and real-time monitoring of Hg fluctuations in organ damage are crucial but limited due to the lack of noninvasive and deep tissue imaging probes. Herein, a Hg-triggered targeted and NIR-II fluorescence/photoacoustic (PA) dual-mode molecular probe (NHG-2) was developed for real-time monitoring Hg fluctuations in Hg-induced acute liver and kidney injury mice.
View Article and Find Full Text PDFPhotoacoustics
February 2025
Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
Recent advances in Light Emitting Diode (LED) technology have enabled a more affordable high frame rate photoacoustic imaging (PA) alternative to traditional laser-based PA systems that are costly and have slow pulse repetition rate. However, a major disadvantage with LEDs is the low energy outputs that do not produce high signal-to-noise ratio (SNR) PA images. There have been recent advancements in integrating deep learning methodologies aimed to address the challenge of improving SNR in LED-PA images, yet comprehensive evaluations across varied datasets and architectures are lacking.
View Article and Find Full Text PDFPhotoacoustics
February 2025
Department of Biomedical Engineering, Eindhoven University of Technology, De Rondom 70, Eindhoven, The Netherlands.
Photoacoustic imaging (PAI) is a developing image modality that benefits from light-matter interaction and low acoustic attenuation to provide functional information on tissue composition at relatively large depths. Several studies have reported the potential of dichroism-sensitive photoacoustic (DS-PA) imaging to expand PAI capabilities by obtaining morphological information of tissue regarding anisotropy and predominant orientation. However, most of these studies have limited their analysis to superficial scanning of samples, where fluence effects are negligible.
View Article and Find Full Text PDFHeliyon
January 2025
Center for MicroElectromechanical Systems (CMEMS), University of Minho, Guimarães, 4800-058, Portugal.
Recently, Organ-on-a-Chip (OoC) platforms have arisen as an increasingly relevant experimental tool for successfully replicating human physiology and disease. However, there is a lack of a standard technology to monitor the OoC parameters, especially in a non-invasive and label-free way. Photoacoustic (PA) systems can be considered an alternative and accurate assessment method for OoC platforms.
View Article and Find Full Text PDFAdv Mater
January 2025
Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, 610064, China.
Breast cancer (BC) is the second most common cause of cancer induced death worldwide. Current statistics has disclosed that the diabetic BC patients have significantly worse survival rate compared with nondiabetic BC patients. However, the specific mechanism is still being explored.
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