Metabolite Variations during the First Weeks of Growth of Immature and by Untargeted Liquid Chromatography-Mass Spectrometry/Mass Spectrometry Metabolomics.

Molecules

Institut National des Sciences Appliquées (INSA) Rouen Normandie, Univ Rouen Normandie, Centre National de la Recherche Scientifique (CNRS), Normandie Univ, Chimie Organique et Bioorganique Réactivité et Analyse (COBRA) UMR 6014, INC3M FR 3038, 76000 Rouen, France.

Published: August 2024

AI Article Synopsis

  • Immature citrus fruits are valuable to the pharmaceutical industry because they contain beneficial metabolites.
  • A study used liquid chromatography-mass spectrometry to analyze the metabolic changes in four citrus cultivars as they grew in size.
  • Results showed significant differences in metabolite profiles between mandarin and orange cultivars, identifying 40 metabolites that increased and 31 that decreased as the fruits grew.

Article Abstract

Immature citruses are an important resource for the pharmaceutical industry due to their high levels of metabolites with health benefits. In this study, we used untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics to investigate the changes associated with fruit size in immature citrus fruits in the first weeks of growth. Three orange cultivars ( 'Navel', 'Valencia', and 'Valencia Late') and a mandarin ( 'Fremont') were separated into eight fruit sizes, extracted, and analyzed. Statistical analyses revealed a distinct separation between the mandarin and the oranges based on 56 metabolites, with an additional separation between the 'Navel' orange and the 'Valencia' and 'Valencia Late' oranges based on 21 metabolites. Then, metabolites that evolved significantly with fruit size growth were identified, including 40 up-regulated and 31 down-regulated metabolites. This study provides new insights into the metabolite modifications of immature and in the first weeks of growth and emphasizes the significance of including early sampled fruits in citrus maturation studies.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11357260PMC
http://dx.doi.org/10.3390/molecules29163718DOI Listing

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