The genus holds a key phylogenetic position as a sister species to vascular plants, encompassing desiccation-tolerant members. Some species thrive in extremely arid conditions, enduring significant water loss and recovering upon rehydration. Consequently, has emerged as a model system for studying desiccation tolerance in plant science. However, the absence of an efficient genetic transformation system has limited the utility of species as a model. To address this constraint, we developed a nanoparticle-mediated transformation tool utilizing arginine-functionalized nanohydroxyapatites. This biocompatible system enabled the transient expression of the , , and reporter genes in . Establishing a stable genetic transformation technique for holds promise for application to other species. This tool could be instrumental in identifying genetic resources for crop improvement and understanding genome-level regulatory mechanisms governing desiccation tolerance in species. Furthermore, this tool might aid in identifying key regulatory genes associated with desiccation tolerance, offering potential applications in enhancing drought-sensitive crops and ensuring sustainable food production.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11353325 | PMC |
| http://dx.doi.org/10.3390/genes15081091 | DOI Listing |
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