Fisch. ex Bunge (syn. Bunge) is one of the notable medicinal and food plants. Therefore, the aim of this study was to calculate the phenolic composition and antioxidant, antimicrobial, as well as enzyme inhibitory [acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR)] activities with chemometric approaches of the hydromethanolic and water extracts of commercial samples. Ten individual phenolic compounds were determined using high-performance liquid chromatography (HPLC), and only quercetin was found at a level of above 80 µg/g DW in both extracts. Moreover, the highest antioxidant activity in DPPH, FRAP, ABTS, and CUPRAC assays was found in the sample containing the roots in loose form from USA. extracts displayed the inhibition zone diameters within the range from 10 to 22 mm antimicrobial activity against , while there were no inhibition zones in any extracts in case of . The extracts of showed an inhibition rate below 40% against TYR, and among tested extracts, only two samples were able to inhibit BChE with IC values of above 30 µg/mL. Correlation analysis showed a highly positive relationship between their phenolic composition and antioxidant activity. Concluding, the obtained results confirmed that commercial samples could be an important dietary source of natural antioxidants.
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http://dx.doi.org/10.3390/antiox13080993 | DOI Listing |
Anal Bioanal Chem
January 2025
Statistical Engineering Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899-8980, USA.
Closely related species of Salmonidae, including Pacific and Atlantic salmon, can be distinguished from one another based on nucleotide sequences from the cytochrome c oxidase sub-unit 1 mitochondrial gene (COI), using ensembles of fragments aligned to genetic barcodes that serve as digital proxies for the relevant species. This is accomplished by exploiting both the nucleotide sequences and their quality scores recorded in a FASTQ file obtained via Next Generation (NextGen) Sequencing of mitochondrial DNA extracted from Coho salmon caught with hook and line in the Gulf of Alaska. The alignment is done using MUSCLE (Muscle 5.
View Article and Find Full Text PDFJ Biophotonics
January 2025
Laser Biomedical Applications Division, Raja Ramanna Centre for Advanced Technology, Indore, India.
Availability of a suitable tool for carrying out non-invasive measurement of Raman signatures in situ, from biological tissues having low Raman cross section is a clinically unmet need faced with manifold challenges. A Raman probe can prove to be an invaluable component of clinical Raman diagnostic systems. We present development of a Raman probe capable of measuring artefact free Raman spectra of biological tissues in situ.
View Article and Find Full Text PDFPharm Res
January 2025
BioDev Department WuXi Biologics USA, 1 Cedarbrook Dr, Cranbury, NJ, 08512, USA.
Background: High concentration protein formulation (HCPF) development needs to balance protein stability attributes such as conformational/colloidal stability, chemical stability, and solution properties such as viscosity and osmolality.
Methodology: A three-phase design is established in this work. In Phase 1, conformational and colloidal stability are measured by 384-well-based high-throughput (HT) biophysical screening while viscosity reduction screening is performed with HT viscosity screening.
NPJ Microgravity
January 2025
NASA John F. Kennedy Space Center, Kennedy Space Center, Merritt Island, FL, USA.
The MISSE-Seed project was designed to investigate the effects of space exposure on seed quality and storage. The project tested the Multipurpose Materials International Space Station Experiment-Flight Facility (MISSE-FF) hardware as a platform for exposing biological samples to the space environment outside the International Space Station (ISS). Furthermore, it evaluated the capability of a newly designed passive sample containment canister as a suitable exposure unit for biological samples for preserving their vigor while exposing to the space environment to study multi-stressor effects.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address:
This study investigated the potential of Maillard reaction products (MRPs) derived from mung bean protein isolate (MBPI) and peach gum (PG) conjugates as wall materials for microencapsulating chia seed oil (CSO). Four formulations (MMRP) were prepared using spray-drying and compared to a commercial sample (CMMRP). The MMRP formulation exhibited the highest encapsulation yield (91 %) and encapsulation efficiency (96 %), along with favorable physical properties, including a spherical shape and smooth surface.
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