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An integrated single-cell reference atlas of the human endometrium. | LitMetric

AI Article Synopsis

  • The Human Endometrial Cell Atlas (HECA) is a comprehensive single-cell reference atlas derived from 313,527 cells, profiling endometrial samples from 63 women, both with and without endometriosis.
  • HECA not only categorizes known cell types but also identifies new ones, utilizing advanced techniques like spatial transcriptomics and validation through an independent single-nuclei dataset.
  • The findings reveal significant cellular interactions in the endometrium, suggest potential dysregulation of specific cell types in endometriosis, and position HECA as a crucial tool for understanding endometrial health and related disorders.

Article Abstract

The complex and dynamic cellular composition of the human endometrium remains poorly understood. Previous endometrial single-cell atlases profiled few donors and lacked consensus in defining cell types. We introduce the Human Endometrial Cell Atlas (HECA), a high-resolution single-cell reference atlas (313,527 cells) combining published and new endometrial single-cell transcriptomics datasets of 63 women with and without endometriosis. HECA assigns consensus and identifies previously unreported cell types, mapped in situ using spatial transcriptomics and validated using a new independent single-nuclei dataset (312,246 nuclei, 63 donors). In the functionalis, we identify intricate stromal-epithelial cell coordination via transforming growth factor beta (TGFβ) signaling. In the basalis, we define signaling between fibroblasts and an epithelial population expressing progenitor markers. Integration of HECA with large-scale endometriosis genome-wide association study data pinpoints decidualized stromal cells and macrophages as most likely dysregulated in endometriosis. The HECA is a valuable resource for studying endometrial physiology and disorders, and for guiding microphysiological in vitro systems development.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11387200PMC
http://dx.doi.org/10.1038/s41588-024-01873-wDOI Listing

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