AI Article Synopsis
- - The control of gene expression is crucial for genetic engineering, especially for manipulating pests like mosquitoes, but current methods lack reliable data in nonmodel insect species.
- - Researchers have found that using RNA polymerase II promoters limits options for gene expression control, prompting the need for new strategies.
- - A new systematic approach was developed to modify translation initiation sequences and 3' UTRs, successfully creating a toolbox that allows for predictable gene expression changes in mosquitoes, enhancing genetic tools for pest management.
Article Abstract
The ability to control gene expression is pivotal in genetic engineering and synthetic biology. However, in most nonmodel and pest insect species, empirical evidence for predictable modulation of gene expression levels is lacking. This knowledge gap is critical for genetic control systems, particularly in mosquitoes, where transgenic methods offer novel routes for pest control. Commonly, the choice of RNA polymerase II promoter (Pol II) is the primary method for controlling gene expression, but the options are limited. To address this, we developed a systematic approach to characterize modifications in translation initiation sequences (TIS) and 3' untranslated regions (UTR) of transgenes, enabling the creation of a toolbox for gene expression modulation in mosquitoes and potentially other insects. The approach demonstrated highly predictable gene expression changes across various cell lines and 5' regulatory sequences, representing a significant advancement in mosquito synthetic biology gene expression tools.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11421086 | PMC |
| http://dx.doi.org/10.1021/acssynbio.4c00250 | DOI Listing |
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