AI Article Synopsis
- Researchers studied growth differences in spotted seabass on a low-phosphorus diet, using 150 fish over eight weeks to identify fast-growing (FG) and slow-growing (SG) categories based on weight.
- Analysis of the fish livers through metabolomics and transcriptomics showed that FG had a higher antioxidant capacity, and 431 differentially expressed genes were linked to metabolism, with many involved in processes like protein digestion and substance transport.
- The study concluded that FG exhibited enhanced liver functions related to immune response and amino acid metabolism, providing insights for improving low-phosphorus diets and selective breeding practices for spotted seabass.
Article Abstract
To analyze the potential mechanisms of growth differences in spotted seabass () fed a low-phosphorus diet, a total of 150 spotted seabass with an initial body weight of 4.49 ± 0.01 g were used (50 fish per tank) and fed a low-phosphorus diet for eight weeks. At the end of the experiment, five of the heaviest and five of the lightest fish were selected from each tank as fast-growing spotted seabass (FG) and slow-growing spotted seabass (SG), respectively, and their livers were analyzed by metabolomics and transcriptomics. The hepatic antioxidant capacity of the FG fed a low-phosphorus diet was significantly higher than that of the SG. A total of 431 differentially expressed genes (DEGs) were determined in the two groups, and most of the DEGs were involved in metabolism-related pathways such as steroid biosynthesis, glycolysis/gluconeogenesis, and protein digestion and absorption. Substance transport-related regulators and transporters were predominantly up-regulated. Furthermore, a large number of metabolites in the liver of FG were significantly up-regulated, especially amino acids, decanoyl-L-carnitine and dehydroepiandrosterone. The integration analysis of differential metabolites and genes further revealed that the interaction between protein digestion and absorption, as well as phenylalanine metabolism pathways were significantly increased in the liver of FG compared to those of the SG. In general, FG fed a low-phosphorus diet induced an enhancement in hepatic immune response, substance transport, and amino acid metabolism. This study provides new information on genetic mechanisms and regulatory pathways underlying differential growth rate and provides a basis for the foundation of efficient utilization of low-phosphorus diets and selective breeding programs for spotted seabass.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11355958 | PMC |
| http://dx.doi.org/10.3390/metabo14080406 | DOI Listing |
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- Analysis of the fish livers through metabolomics and transcriptomics showed that FG had a higher antioxidant capacity, and 431 differentially expressed genes were linked to metabolism, with many involved in processes like protein digestion and substance transport.
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